H-Ile-Pro-Pro-OH hydrochloride

Name: H-Ile-Pro-Pro-OH hydrochloride
Brand: MedChemExpress
SKU: HY-114424A
Rating: 4.5 (1 reviews)

Cat. No.: HY-114424A Purity: 98.48%: FAQs
Data Sheet SDS COA Handling Instructions

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H-Ile-Pro-Pro-OH hydrochloride, a milk-derived peptide, inhibits angiotensin-converting enzyme (ACE) with an IC₅₀ of 5 μM. Antihypertensive tripeptides.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

H-Ile-Pro-Pro-OH hydrochloride Chemical Structure

CAS No. : 1208862-61-6

Size	Price	Stock	Quantity
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of H-Ile-Pro-Pro-OH hydrochloride:

H-Ile-Pro-Pro-OH Get quote

1 Publications Citing Use of MCE H-Ile-Pro-Pro-OH hydrochloride

•J Physiol Biochem. 2024 Jul 15. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

H-Ile-Pro-Pro-OH hydrochloride, a milk-derived peptide^[1], inhibits angiotensin-converting enzyme (ACE)^[1] with an IC₅₀ of 5 μM^[2]. Antihypertensive tripeptides^[1].

IC₅₀ & Target

IC50: 5 μM (ACE)^[2]

In Vitro

Ile-Pro-Pro (IPP; 1 nM, 0.1 μM, and 10 μM) increases nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ile-Pro-Pro (IPP; 30 mg/kg per day; 0.3 g/L) attenuates arterial dysfunction in L-NAME-treated rats^[1]
Ile-Pro-Pro decreases blood pressure in spontaneously hypertensive rats (SHR) ^[1].
Ile-Pro-Pro attenuates the development of atherosclerosis in apolipoprotein E-deficient (apoE(-/-)) mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

361.86

Formula

C₁₆H₂₈ClN₃O₄

CAS No.

1208862-61-6

Appearance

Solid

Color

Off-white to light yellow

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 55 mg/mL (151.99 mM; Need ultrasonic)

DMSO : 36.67 mg/mL (101.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7635 mL	13.8175 mL	27.6350 mL
5 mM	0.5527 mL	2.7635 mL	5.5270 mL
10 mM	0.2763 mL	1.3817 mL	2.7635 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (7.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (7.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (7.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (276.35 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.48%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (253 KB) HNMR (273 KB) RP-HPLC (263 KB) MS (368 KB) Elemental Analysis Report (114 KB)

Handling Instructions (2659 KB)

References

[1]. Nonaka A, et al. The milk-derived peptides Val-Pro-Pro and Ile-Pro-Pro attenuate arterial dysfunction in L-NAME-treated rats. Hypertens Res. 2014 Aug;37(8):703-7. [Content Brief]

[2]. Nakamura Y, et al. Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk. J Dairy Sci. 1995 Apr;78(4):777-83. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.7635 mL	13.8175 mL	27.6350 mL	69.0875 mL
	5 mM	0.5527 mL	2.7635 mL	5.5270 mL	13.8175 mL
	10 mM	0.2763 mL	1.3817 mL	2.7635 mL	6.9087 mL
	15 mM	0.1842 mL	0.9212 mL	1.8423 mL	4.6058 mL
	20 mM	0.1382 mL	0.6909 mL	1.3817 mL	3.4544 mL
	25 mM	0.1105 mL	0.5527 mL	1.1054 mL	2.7635 mL
	30 mM	0.0921 mL	0.4606 mL	0.9212 mL	2.3029 mL
	40 mM	0.0691 mL	0.3454 mL	0.6909 mL	1.7272 mL
	50 mM	0.0553 mL	0.2763 mL	0.5527 mL	1.3817 mL
	60 mM	0.0461 mL	0.2303 mL	0.4606 mL	1.1515 mL
	80 mM	0.0345 mL	0.1727 mL	0.3454 mL	0.8636 mL
	100 mM	0.0276 mL	0.1382 mL	0.2763 mL	0.6909 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.