2. Metabolic Enzyme/Protease
  3. Cytochrome P450
  4. hCYP3A4 Fluorogenic substrate 1

hCYP3A4 Fluorogenic substrate 1 

Cat. No.: HY-155002 Purity: 99.27%
COA Handling Instructions

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hCYP3A4 Fluorogenic substrate 1 is a potrnt hCYP3A4 fluorogenic substrate with an Km value of 0.36 μM. hCYP3A4 Fluorogenic substrate 1 can be used for cell and in vivo imaging.

For research use only. We do not sell to patients.

hCYP3A4 Fluorogenic substrate 1 Chemical Structure

hCYP3A4 Fluorogenic substrate 1 Chemical Structure

CAS No. : 186299-00-3

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5 mg USD 150 In-stock
10 mg USD 240 In-stock
25 mg USD 480 In-stock
50 mg USD 760 In-stock
100 mg USD 1250 In-stock
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Based on 1 publication(s) in Google Scholar

hCYP3A4 Fluorogenic substrate 1 Related Antibodies

Top Publications Citing Use of Products

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms
CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

hCYP3A4 Fluorogenic substrate 1 is a potrnt hCYP3A4 fluorogenic substrate with an Km value of 0.36 μM. hCYP3A4 Fluorogenic substrate 1 can be used for cell and in vivo imaging[1].

IC50 & Target[1]

CYP3A4

0.36 μM (Km)

In Vitro

hCYP3A4 Fluorogenic substrate 1 (compound F8) (20 μM; 30 min) shows brightly fluorescent signals at the green channel in both living Hep3B2 cells and Huh-7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

hCYP3A4 Fluorogenic substrate 1 Related Antibodies
In Vivo

hCYP3A4 Fluorogenic substrate 1 (10 mg/kg; i.v.) can be used for imaging in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats[1]
Dosage: 10 mg/kg
Administration: I.v.
Result: Can be used as a probe substrate offers a highly efficient and easy-to-use approach for screening and characterizing hCYP3A4 inhibitors or time-dependent inactivators, which strongly facilitates hCYP3A4-mediated DDI studies.
Molecular Weight

305.30

Formula

C19H12FNO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C2C(C3=O)=CC=CC2=CC=C1)N3CC4=CC=C(F)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (40.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2755 mL 16.3773 mL 32.7547 mL
5 mM 0.6551 mL 3.2755 mL 6.5509 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2755 mL 16.3773 mL 32.7547 mL 81.8867 mL
5 mM 0.6551 mL 3.2755 mL 6.5509 mL 16.3773 mL
10 mM 0.3275 mL 1.6377 mL 3.2755 mL 8.1887 mL
15 mM 0.2184 mL 1.0918 mL 2.1836 mL 5.4591 mL
20 mM 0.1638 mL 0.8189 mL 1.6377 mL 4.0943 mL
25 mM 0.1310 mL 0.6551 mL 1.3102 mL 3.2755 mL
30 mM 0.1092 mL 0.5459 mL 1.0918 mL 2.7296 mL
40 mM 0.0819 mL 0.4094 mL 0.8189 mL 2.0472 mL
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hCYP3A4 Fluorogenic substrate 1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

hCYP3A4 Fluorogenic substrate 1186299-00-3Cytochrome P450CYPsInhibitorinhibitorinhibit

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