2. Anti-infection
  3. HBV
  4. Helioxanthin derivative 5-4-2

Helioxanthin derivative 5-4-2  (Synonyms: Helioxanthin 5-4-2)

Cat. No.: HY-16679 Purity: 99.80%
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Helioxanthin derivative 5-4-2 is an analogue of helioxanthin, exhibites significant in vitro anti-HBV activity with EC50 of 0.

For research use only. We do not sell to patients.

Helioxanthin derivative 5-4-2 Chemical Structure

Helioxanthin derivative 5-4-2 Chemical Structure

CAS No. : 203935-39-1

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1 mg USD 240 In-stock
5 mg USD 720 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Helioxanthin derivative 5-4-2:

Helioxanthin derivative 5-4-2 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Helioxanthin derivative 5-4-2

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  • Purity & Documentation

  • References

  • Customer Review

Description

Helioxanthin derivative 5-4-2 is an analogue of helioxanthin, exhibites significant in vitro anti-HBV activity with EC50 of 0.08 uM in HepG2.2.15 cells. IC50 value: 0.08 uM (EC50) [1][2] Target: Anti-HBV Helioxanthin derivative 5-4-2 had potent anti-HBV activities in HepG2.2.15 cells, with the EC50s of 1 and 0.08 microM, respectively. The lamivudine-resistant HBV, L526M/M550V double mutant strain, was also sensitive to helioxanthin and 5-4-2. This class of compounds not only inhibited HBV DNA, but also decreased HBV mRNA and HBV protein expression. The EC50 of HBV DNA inhibition was consistent with the EC50 of HBV 3.5 Kb transcript inhibition, which was 1 and 0.09 microM for helioxanthin and 5-4-2 respectively.

Cellular Effect
Cell Line Type Value Description References
HepG2 IC50
> 1 μM
Compound: 38444
Inhibition of HBV promoter in human HepG2 cells by luciferase reporter gene assay
Inhibition of HBV promoter in human HepG2 cells by luciferase reporter gene assay
[PMID: 17488817]
Molecular Weight

347.32

Formula

C20H13NO5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1NCC2=C(C3=CC=C(OCO4)C4=C3)C5=C6C(OCO6)=CC=C5C=C21
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (143.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.80%

SDS (252 KB)

COA (260 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8792 mL 14.3959 mL 28.7919 mL 71.9797 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL 14.3959 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL 7.1980 mL
15 mM 0.1919 mL 0.9597 mL 1.9195 mL 4.7986 mL
20 mM 0.1440 mL 0.7198 mL 1.4396 mL 3.5990 mL
25 mM 0.1152 mL 0.5758 mL 1.1517 mL 2.8792 mL
30 mM 0.0960 mL 0.4799 mL 0.9597 mL 2.3993 mL
40 mM 0.0720 mL 0.3599 mL 0.7198 mL 1.7995 mL
50 mM 0.0576 mL 0.2879 mL 0.5758 mL 1.4396 mL
60 mM 0.0480 mL 0.2399 mL 0.4799 mL 1.1997 mL
80 mM 0.0360 mL 0.1799 mL 0.3599 mL 0.8997 mL
100 mM 0.0288 mL 0.1440 mL 0.2879 mL 0.7198 mL
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Helioxanthin derivative 5-4-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Helioxanthin derivative 5-4-2203935-39-1Helioxanthin 5-4-2HBVHepatitis B virusInhibitorinhibitorinhibit

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