Hexamethonium Bromide

Name: Hexamethonium Bromide
Brand: MedChemExpress
SKU: HY-B0569
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0569 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hexamethonium Bromide is a non-selective ganglionic nicotinic-receptor antagonist (nAChR) antagonist, with mixed competitive and noncompetitive activity. Hexamethonium Bromide has anti-hypertensive activity. Hexamethonium Bromide attenuates sympathetic activity and blood pressure in spontaneously hypertensive animal models.

For research use only. We do not sell to patients.

Hexamethonium Bromide Chemical Structure

CAS No. : 55-97-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
5 g	USD 60	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Hexamethonium Bromide:

Hexamethonium chloride Get quote

3 Publications Citing Use of MCE Hexamethonium Bromide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

nAChR^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
J774.1	IC₅₀	> 100 μM Compound: 3d	Cytotoxicity against mouse J774.1 cells after 48 hrs by alamar blue satining Cytotoxicity against mouse J774.1 cells after 48 hrs by alamar blue satining	[PMID: 20382538]

In Vitro

Hexamethonium Bromide (100 μM; 60 minutes) abolishes epibatidine-induced depolarisations of smooth muscle cells^[1].
The α3β4α5 receptors have greater sensitivity to Hexamethonium Bromide than the other potential ganglionic models, α3β4, α3β2, and α3β2α5^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Hexamethonium Bromide (0.2-25 mg/kg; i.v.) significantly reduces the renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) in the Wistar rats and the spontaneously hypertensive rats (SHRs)^[4].
Hexamethonium Bromide (0.2-1.0 mg/kg; i.v.) treatments show no significant differences in the RSNA, MAP or HR between Wistar rats and SHRs^[4].
Hexamethonium Bromide (5.0-25 mg/kg; i.v.) results in a greater reduction in the RSNA and MAP in SHRs compared with Wistar rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male normotensive Wistar rats (280-320 g), SHRs^[4]
Dosage:	0.2 mg/kg, 1.0 mg/kg, 5.0 mg/kg, 25 mg/kg
Administration:	Intravenous injection
Result:	Significantly reduced the RSNA, MAP and HR in the Wistar rats and the SHRs.

Molecular Weight

362.19

Formula

C₁₂H₃₀Br₂N₂

CAS No.

55-97-0

Appearance

Solid

Color

White to off-white

SMILES

C[N+](C)(C)CCCCCC[N+](C)(C)C.[Br-].[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (276.10 mM)

DMSO : 16.67 mg/mL (46.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7610 mL	13.8049 mL	27.6098 mL
5 mM	0.5522 mL	2.7610 mL	5.5220 mL
10 mM	0.2761 mL	1.3805 mL	2.7610 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (4.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (276.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (418 KB)

COA (242 KB) HNMR (252 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Damian J. Williams, et al. Mechanisms involved in nicotinic acetylcholine receptor-induced neurotransmitter release from sympathetic nerve terminals in the mouse vas deferens. PLoS One. 2011; 6(12): e29209. [Content Brief]

[2]. Roger L. Papke, et al. Activation and Inhibition of Mouse Muscle and Neuronal Nicotinic Acetylcholine Receptors Expressed in Xenopus Oocytes. J Pharmacol Exp Ther. 2010 May; 333(2): 501–518. [Content Brief]

[3]. Brian T Hawkins, et al. Modulation of cerebral microvascular permeability by endothelial nicotinic acetylcholine receptors. Am J Physiol Heart Circ Physiol. 2005 Jul;289(1):H212-9. [Content Brief]

[4]. Peng Li, et al. Hexamethonium attenuates sympathetic activity and blood pressure in spontaneously hypertensive rats. Mol Med Rep. 2015 Nov;12(5):7116-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.7610 mL	13.8049 mL	27.6098 mL	69.0245 mL
	5 mM	0.5522 mL	2.7610 mL	5.5220 mL	13.8049 mL
	10 mM	0.2761 mL	1.3805 mL	2.7610 mL	6.9025 mL
	15 mM	0.1841 mL	0.9203 mL	1.8407 mL	4.6016 mL
	20 mM	0.1380 mL	0.6902 mL	1.3805 mL	3.4512 mL
	25 mM	0.1104 mL	0.5522 mL	1.1044 mL	2.7610 mL
	30 mM	0.0920 mL	0.4602 mL	0.9203 mL	2.3008 mL
	40 mM	0.0690 mL	0.3451 mL	0.6902 mL	1.7256 mL
H₂O	50 mM	0.0552 mL	0.2761 mL	0.5522 mL	1.3805 mL
	60 mM	0.0460 mL	0.2301 mL	0.4602 mL	1.1504 mL
	80 mM	0.0345 mL	0.1726 mL	0.3451 mL	0.8628 mL
	100 mM	0.0276 mL	0.1380 mL	0.2761 mL	0.6902 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.