Hydroxytyrosol acetate

Name: Hydroxytyrosol acetate
Brand: MedChemExpress
SKU: HY-N6043
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6043 Purity: 98.56%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hydroxytyrosol acetate is found in the olive oil with an antioxidant activity. Hydroxytyrosol acetate had a weaker DPPH radical scavenging activity than hydroxytyrosol.

For research use only. We do not sell to patients.

Hydroxytyrosol acetate Chemical Structure

CAS No. : 69039-02-7

Size	Price	Stock	Quantity
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Hydroxytyrosol acetate is found in the olive oil with an antioxidant activity. Hydroxytyrosol acetate had a weaker DPPH radical scavenging activity than hydroxytyrosol^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	32 μM Compound: 1	Cytotoxicity against human Caco2/TC7 cells assessed as growth inhibition after 24 hrs by WST-1 assay Cytotoxicity against human Caco2/TC7 cells assessed as growth inhibition after 24 hrs by WST-1 assay	[PMID: 26225717]
HEp-2	CC₅₀	50 μg/mL Compound: 4a	Cytotoxicity against human Hep2 cells after 24 hrs by MTT assay Cytotoxicity against human Hep2 cells after 24 hrs by MTT assay	[PMID: 26260341]
HFF-1	CC₅₀	50 μg/mL Compound: 4a	Cytotoxicity against human HFF1 cells after 24 hrs by MTT assay Cytotoxicity against human HFF1 cells after 24 hrs by MTT assay	[PMID: 26260341]
MRC5	IC₅₀	> 50 μM Compound: 10	Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 27155468]
Platelet	IC₅₀	10 μM Compound: 1	Inhibition of collagen-induced platelet aggregation in human whole blood preincubated for 10 mins prior to collagen addition measured after 10 mins by aggregometric analysis Inhibition of collagen-induced platelet aggregation in human whole blood preincubated for 10 mins prior to collagen addition measured after 10 mins by aggregometric analysis	[PMID: 26225717]
THP-1	IC₅₀	> 10 μM Compound: 10	Antileishmanial activity against Leishmania donovani MHOM/ET/67/HU3 intracellular amastigotes infected in human THP1 cells after 72 hrs by luciferase coupled luminescence assay Antileishmanial activity against Leishmania donovani MHOM/ET/67/HU3 intracellular amastigotes infected in human THP1 cells after 72 hrs by luciferase coupled luminescence assay	[PMID: 27155468]
Vero	CC₅₀	100 μg/mL Compound: 4a	Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay	[PMID: 26260341]

Molecular Weight

196.20

Formula

C₁₀H₁₂O₄

CAS No.

69039-02-7

Appearance

Solid

Color

White to off-white

SMILES

CC(OCCC1=CC(O)=C(O)C=C1)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1274.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0968 mL	25.4842 mL	50.9684 mL
5 mM	1.0194 mL	5.0968 mL	10.1937 mL
10 mM	0.5097 mL	2.5484 mL	5.0968 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (31.86 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (31.86 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 6.25 mg/mL (31.86 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.56%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (247 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Gordon MH, et al. Antioxidant activity of hydroxytyrosol acetate compared with that of other olive oil polyphenols.J Agric Food Chem. 2001 May;49(5):2480-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.0968 mL	25.4842 mL	50.9684 mL	127.4210 mL
	5 mM	1.0194 mL	5.0968 mL	10.1937 mL	25.4842 mL
	10 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	15 mM	0.3398 mL	1.6989 mL	3.3979 mL	8.4947 mL
	20 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	25 mM	0.2039 mL	1.0194 mL	2.0387 mL	5.0968 mL
	30 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	40 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	50 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	60 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	80 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	100 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL