Indimitecan (Synonyms: LMP776)

Name: Indimitecan
Brand: MedChemExpress
SKU: HY-18350
Rating: 4.5 (1 reviews)

Cat. No.: HY-18350 Purity: 98.03%: FAQs
Data Sheet SDS COA Handling Instructions

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Indimitecan (LMP776) is a topoisomerase I (Top1) inhibitor with anticancer activities.

For research use only. We do not sell to patients.

Indimitecan Chemical Structure

CAS No. : 915360-05-3

Size	Price	Stock	Quantity
5 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 910	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1280	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Indimitecan:

Indimitecan hydrochloride Get quote

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Topoisomerase Topo I Topo II

Biological Activity
Purity & Documentation
References
Customer Review

Description

Indimitecan (LMP776) is a topoisomerase I (Top1) inhibitor with anticancer activities^[1].

IC₅₀ & Target

Top1

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	GI₅₀	< 0.01 μM Compound: 7, indimitecan	Antiproliferative activity against human DU145 cells by SRB assay Antiproliferative activity against human DU145 cells by SRB assay	[PMID: 21710981]
HCT-116	GI₅₀	< 0.01 μM Compound: 7, indimitecan	Antiproliferative activity against human HCT116 cells by SRB assay Antiproliferative activity against human HCT116 cells by SRB assay	[PMID: 21710981]
HOP-62	GI₅₀	< 0.01 μM Compound: 7, indimitecan	Antiproliferative activity against human HOP62 cells by SRB assay Antiproliferative activity against human HOP62 cells by SRB assay	[PMID: 21710981]
L1210	EC₅₀	0.06 μM Compound: 19	Trypanocidal activity against Trypanosoma brucei brucei 427 bloodstream forms infected in mouse L1210 cells after 48 hrs Trypanocidal activity against Trypanosoma brucei brucei 427 bloodstream forms infected in mouse L1210 cells after 48 hrs	[PMID: 18824603]
MCF7	GI₅₀	0.01 μM Compound: 7, indimitecan	Antiproliferative activity against human MCF7 cells by SRB assay Antiproliferative activity against human MCF7 cells by SRB assay	[PMID: 21710981]
OVCAR-3	GI₅₀	0.085 μM Compound: 7, indimitecan	Antiproliferative activity against human OVCAR3 cells by SRB assay Antiproliferative activity against human OVCAR3 cells by SRB assay	[PMID: 21710981]
SF-539	GI₅₀	0.037 μM Compound: 7, indimitecan	Antiproliferative activity against human SF539 cells by SRB assay Antiproliferative activity against human SF539 cells by SRB assay	[PMID: 21710981]
SN12C	GI₅₀	< 0.01 μM Compound: 7, indimitecan	Antiproliferative activity against human SN12C cells by SRB assay Antiproliferative activity against human SN12C cells by SRB assay	[PMID: 21710981]
UACC-62	GI₅₀	< 0.01 μM Compound: 7, indimitecan	Antiproliferative activity against human UACC62 cells by SRB assay Antiproliferative activity against human UACC62 cells by SRB assay	[PMID: 21710981]

In Vitro

Indimitecan (LMP776) (0-100 μM) shows antiproliferative potencies against various human cancer cell lines, with mean-graph midpoint (MGM) of 0.079 ± 0.023 μM^[1].
Indimitecan shows potent DNA cleavage due to Top1 inhibition^[1].
Indimitecan can be substrates for metabolic ketone reductases^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	HOP-62, HCT-116, SF-539, UACC-62, OVCAR-3, SN12C, DU-145, MCF-7
Concentration:	0-100 μM
Incubation Time:
Result:	Showed growth inhibition with GI₅₀s of <0.01, <0.01, 0.04, <0.01, 0.08, <0.01, <0.01 and 0.01 μM against HOP-62, HCT-116, SF-539, UACC-62, OVCAR-3, SN12C, DU-145 and MCF-7 cells, respectively. And the mean-graph midpoint (MGM) for growth inhibition of all human cancer cell lines successfully tested (the National Cancer Institute’s developmental therapeutics assay (the “NCI60”)) was 0.079 ± 0.023 μM.

Clinical Trial

Molecular Weight

459.45

Formula

C₂₅H₂₁N₃O₆

CAS No.

915360-05-3

Appearance

Solid

Color

Brown to black

SMILES

O=C1C2=CC(OC)=C(OC)C=C2C3=C(C(C=C(OCO4)C4=C5)=C5C3=O)N1CCCN6C=NC=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 8.33 mg/mL (18.13 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1765 mL	10.8826 mL	21.7652 mL
5 mM	0.4353 mL	2.1765 mL	4.3530 mL
10 mM	0.2177 mL	1.0883 mL	2.1765 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.03%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (258 KB) HNMR (268 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Cinelli MA, et al. Identification, synthesis, and biological evaluation of metabolites of the experimental cancer treatment drugs indotecan (LMP400) and indimitecan (LMP776) and investigation of isomerically hydroxylated indenoisoquinoline analogues as to [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1765 mL	10.8826 mL	21.7652 mL	54.4129 mL
	5 mM	0.4353 mL	2.1765 mL	4.3530 mL	10.8826 mL
	10 mM	0.2177 mL	1.0883 mL	2.1765 mL	5.4413 mL
	15 mM	0.1451 mL	0.7255 mL	1.4510 mL	3.6275 mL

Indimitecan Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indimitecan915360-05-3LMP776LMP 776LMP-776TopoisomeraseanticancerDNA cleavageInhibitorinhibitorinhibit

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Indimitecan (Synonyms: LMP776)

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Other Forms of Indimitecan:

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Indimitecan Related Antibodies

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Complete Stock Solution Preparation Table

Indimitecan Related Classifications

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