Basement Membrane Matrix GFR&HC

Cat. No.: HY-K6009: FAQs
Manual SDS

MCE Basement Membrane Matrix GFR&HC is primarily composed of natural basement membrane matrix extracted from mouse tumors, is suitable for cell cultures that need to reduce growth factor induction and studies related to growth factors, signaling pathways, etc.

Size	Price	Stock	Quantity
Free Sample (1 mL)		Apply Now
5 mL	USD 350	In-stock	Estimated Time of Arrival: December 31
10 mL	USD 637	In-stock	Estimated Time of Arrival: December 31

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Description
Storage
Protocol
Components
Documentation

Description
& Advantages

MCE Basement Membrane Matrix GFR&HC is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is lower than that of standard basement membrane matrix.

MCE Basement Membrane Matrix GFR&HC reduces the background signal induced by growth factors and is suitable for cell cultures that need to reduce growth factor induction and studies related to growth factors, signaling pathways, etc.

Storage

-20°C, 2 years.

Avoid repeated freezing and thawing.

Protocol

a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

b. Dilute MCE Basement Membrane Matrix GFR&HC with cell suspension at 4°C in a 1:1 ratio to a suitable final concentration.

c. Inject diluted cell suspension at a depth of 1 cm under the skin on nude mice. The cell suspension should be injected in 30 minutes and be placed on ice to slow down apoptosis and prevent from gelling.

d. Formed tumors can be seen in about 1 week.

Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.

Components

Components	HY-K6009-5 mL	HY-K6009-10 mL
Basement Membrane Matrix GFR&HC	5 mL	10 mL

Documentation

Manual (224 KB) SDS (532 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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