Basement Membrane Matrix HC (Phenol Red)

Cat. No.: HY-K6005: FAQs
Manual SDS

MCE Basement Membrane Matrix HC (Phenol Red) is primarily composed of natural basement membrane matrix extracted from mouse tumors, with higher content of various cytokines than the standard type.This product is mainly used for angiogenesis experiments, construction of animal models and 3D tumor models.

Size	Price	Stock	Quantity
Free Sample (1 mL)		Apply Now
5 mL	USD 304	In-stock	Estimated Time of Arrival: December 31
10 mL	USD 554	In-stock	Estimated Time of Arrival: December 31

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Basement Membrane Matrix HC (Phenol Red) Featured Recommendations:

MCE Biological Validation

Tumor formation assays

: Basement Membrane Matrix HC (Phenol Red) (HY-K6005) (HY-K6005:HCT-116 cell suspension = 1:1; s.c. a single dose) promotes the tumor formation (HCT-116 subcutaneous xenograft) in 4-5 week old BALB/c-nu nude mice.

: Basement Membrane Matrix HC (Phenol Red) (HY-K6005) (HY-K6005:HCT-116 cell suspension = 1:1; s.c. a single dose) promotes the tumor formation (HCT-116 subcutaneous xenograft) in 4-5 week old BALB/c-nu nude mice. Mice are dissected on day 11 and the tumors size are measured.

Description
Storage
Protocol
Components
Documentation

Description
& Advantages

MCE Basement Membrane Matrix HC (Phenol Red) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is higher than that of standard basement membrane matrix.

MCE Basement Membrane Matrix HC (Phenol Red) is mainly used for angiogenesis experiments, animal models and 3D tumor model construction.

Storage

-20°C, 2 years.

Avoid repeated freezing and thawing.

Protocol

a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

b. Dilute MCE Basement Membrane Matrix HC with cell suspension at 4°C in a 1:1 ratio to a suitable final concentration.

c. Inject diluted cell suspension at a depth of 1 cm under the skin on nude mice. The cell suspension should be injected in 30 minutes and be placed on ice to slow down apoptosis and prevent from gelling.

d. Formed tumors can be seen in about 1 week.

Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.

Components

Components	HY-K6005-5 mL	HY-K6005-10 mL
Basement Membrane Matrix HC (Phenol Red)	5 mL	5 mL ×2

Documentation

Manual (158 KB) SDS (105 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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