2. MAPK/ERK Pathway
  3. JNK
  4. IQ-3

IQ-3 is a specific inhibitor of the c-Jun N-terminal kinase (JNK) family, with preference for JNK3. IQ-3 exhibits Kd values of 0.24 μM, 0.29 μM and 0.066 μM for JNK1, JNK2 and JNK3, respectively.

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IQ-3 Chemical Structure

IQ-3 Chemical Structure

CAS No. : 312538-03-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 109 In-stock
Solution
10 mM * 1 mL in DMSO USD 109 In-stock
Solid
5 mg USD 99 In-stock
10 mg USD 154 In-stock
25 mg USD 308 In-stock
50 mg USD 495 In-stock
100 mg USD 825 In-stock
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Based on 2 publication(s) in Google Scholar

IQ-3 Related Antibodies

Top Publications Citing Use of Products

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JNK1 JNK2 JNK3 JNK

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

IQ-3 is a specific inhibitor of the c-Jun N-terminal kinase (JNK) family, with preference for JNK3. IQ-3 exhibits Kd values of 0.24 μM, 0.29 μM and 0.066 μM for JNK1, JNK2 and JNK3, respectively[1].

IC50 & Target[1]

JNK3

0.066 μM (Kd)

JNK1

0.24 μM (Kd)

JNK2

0.29 μM (Kd)

CK1δ

0.56 μM (Kd)

PI3Kγ

0.43 μM (Kd)

MKNK2

1.2 μM (Kd)

In Vitro

IQ-3 exhibits IC50 of 2.2 μM (TNF-α in human monoMac-6 cells), 1.5 μM (IL-6 in human monoMac-6 cells), 4.7 μM (TNF-α in human PBMCs), 9.1 μM (IL-6 in human PBMCs) and 6.1 μM (NO in murine J774.A1), respectively[1].
IQ-3 exhibits an IC50 of 1.4 μM for inhibiting LPS-induced NF-κB/AP-1 transcriptional activity in human THP-1 Blue monocytic cells[1].
IQ-3 is indeed a competitive inhibitor for the ATP binding site of JNK3[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IQ-3 Related Antibodies

Cell Viability Assay[1]

Cell Line: PBMCs.
Concentration: 0-80 μM (200 ng/mL LPS).
Incubation Time: 30 min.
Result: Downregulated TNF-α concentration (IC50 = 4.7 μM).
Molecular Weight

341.32

Formula

C20H11N3O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(O/N=C1C2=C(C3=NC4=CC=CC=C4N=C3\1)C=CC=C2)C5=CC=CO5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (73.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9298 mL 14.6490 mL 29.2980 mL
5 mM 0.5860 mL 2.9298 mL 5.8596 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 1.25 mg/mL (3.66 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.38%

COA (256 KB) HNMR (266 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9298 mL 14.6490 mL 29.2980 mL 73.2450 mL
5 mM 0.5860 mL 2.9298 mL 5.8596 mL 14.6490 mL
10 mM 0.2930 mL 1.4649 mL 2.9298 mL 7.3245 mL
15 mM 0.1953 mL 0.9766 mL 1.9532 mL 4.8830 mL
20 mM 0.1465 mL 0.7325 mL 1.4649 mL 3.6623 mL
25 mM 0.1172 mL 0.5860 mL 1.1719 mL 2.9298 mL
30 mM 0.0977 mL 0.4883 mL 0.9766 mL 2.4415 mL
40 mM 0.0732 mL 0.3662 mL 0.7325 mL 1.8311 mL
50 mM 0.0586 mL 0.2930 mL 0.5860 mL 1.4649 mL
60 mM 0.0488 mL 0.2442 mL 0.4883 mL 1.2208 mL
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IQ-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IQ-3312538-03-7IQ3IQ 3JNKc-Jun N-terminal kinaseTNF-αIL-6IL-1αIL-1βInhibitorinhibitorinhibit

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