Isopropyl ferulate

Name: Isopropyl ferulate
Brand: MedChemExpress
SKU: HY-N4203
Rating: 4.5 (1 reviews)

Cat. No.: HY-N4203 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Isopropyl ferulate, isolated from Notopterygium incisum, is used in the reduction of pharmaceuticals, preparation of antifungal agents, cosmetics and as antioxidant agent and so forth.

For research use only. We do not sell to patients.

Isopropyl ferulate Chemical Structure

CAS No. : 59831-94-6

Size	Price	Stock	Quantity
1 mg	USD 23	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Isopropyl ferulate, isolated from Notopterygium incisum, is used in the reduction of pharmaceuticals, preparation of antifungal agents, cosmetics and as antioxidant agent and so forth^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
Calu-1	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
HeLa	IC₅₀	31.1 μM Compound: 1d	Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay	[PMID: 22954735]
HOP-62	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
LOX IMVI	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay	[PMID: 22954735]
M14	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H1299	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H157	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H1792	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay	[PMID: 22954735]
NCI-H460	IC₅₀	> 50 μM Compound: 1d	Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay	[PMID: 22954735]

Molecular Weight

236.26

Formula

C₁₃H₁₆O₄

CAS No.

59831-94-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(OC(C)C)/C=C/C1=CC=C(O)C(OC)=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (423.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2326 mL	21.1631 mL	42.3263 mL
5 mM	0.8465 mL	4.2326 mL	8.4653 mL
10 mM	0.4233 mL	2.1163 mL	4.2326 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (269 KB) SDS (251 KB)

COA (245 KB) HNMR (249 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Kumar A, et al. Synthesis of isopropyl ferulate using silica-immobilized lipase in an organic medium. Enzyme Res. 2011;2011:718949. [Content Brief]

[2]. Li F, et al. Anti-austerity agents from Rhizoma et Radix Notopterygii (Qianghuo). Planta Med. 2012 May;78(8):796-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2326 mL	21.1631 mL	42.3263 mL	105.8156 mL
	5 mM	0.8465 mL	4.2326 mL	8.4653 mL	21.1631 mL
	10 mM	0.4233 mL	2.1163 mL	4.2326 mL	10.5816 mL
	15 mM	0.2822 mL	1.4109 mL	2.8218 mL	7.0544 mL
	20 mM	0.2116 mL	1.0582 mL	2.1163 mL	5.2908 mL
	25 mM	0.1693 mL	0.8465 mL	1.6931 mL	4.2326 mL
	30 mM	0.1411 mL	0.7054 mL	1.4109 mL	3.5272 mL
	40 mM	0.1058 mL	0.5291 mL	1.0582 mL	2.6454 mL
	50 mM	0.0847 mL	0.4233 mL	0.8465 mL	2.1163 mL
	60 mM	0.0705 mL	0.3527 mL	0.7054 mL	1.7636 mL
	80 mM	0.0529 mL	0.2645 mL	0.5291 mL	1.3227 mL
	100 mM	0.0423 mL	0.2116 mL	0.4233 mL	1.0582 mL