2. Immunology/Inflammation
  3. NO Synthase
  4. Isosorbide dinitrate

Isosorbide dinitrate  (Synonyms: ISDN)

Cat. No.: HY-B1409 Purity: 99.89%
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Isosorbide dinitrate (ISDN) is an NO donor that prevents LV remodeling and degradation of cardiac function following myocardial infarction (MI).

For research use only. We do not sell to patients.

Isosorbide dinitrate Chemical Structure

Isosorbide dinitrate Chemical Structure

CAS No. : 87-33-2

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10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 33 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Isosorbide dinitrate:

Isosorbide dinitrate Related Antibodies

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  • Purity & Documentation

  • References

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Description

Isosorbide dinitrate (ISDN) is an NO donor that prevents LV remodeling and degradation of cardiac function following myocardial infarction (MI)[1].

In Vivo

Isosorbide dinitrate (3 mg/kg; intratracheal; for 13 days) improves pulmonary artery pressure and ventricular remodeling in a rat model of heart failure following myocardial infarction[1].
Isosorbide dinitrate postconditioning exhibits a cardioprotective effect against rat myocardial ischemia-reperfusion injury in vivo[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5–6 weeks old male juvenile Sprague-Dawley rats (200–250 g)[1]
Dosage: 3 mg/kg
Administration: Intratracheal; for 13 days following coronary ligation
Result: Reduced MI size and alleviated left and right ventricular remodeling following MI.
Clinical Trial
Molecular Weight

236.14

Formula

C6H8N2O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[H][C@]12OC[C@H](O[N+]([O-])=O)[C@]1(OC[C@H]2O[N+]([O-])=O)[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (423.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2348 mL 21.1739 mL 42.3478 mL
5 mM 0.8470 mL 4.2348 mL 8.4696 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

COA (255 KB) HNMR (143 KB) RP-HPLC (79 KB) LCMS (86 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2348 mL 21.1739 mL 42.3478 mL 105.8694 mL
5 mM 0.8470 mL 4.2348 mL 8.4696 mL 21.1739 mL
10 mM 0.4235 mL 2.1174 mL 4.2348 mL 10.5869 mL
15 mM 0.2823 mL 1.4116 mL 2.8232 mL 7.0580 mL
20 mM 0.2117 mL 1.0587 mL 2.1174 mL 5.2935 mL
25 mM 0.1694 mL 0.8470 mL 1.6939 mL 4.2348 mL
30 mM 0.1412 mL 0.7058 mL 1.4116 mL 3.5290 mL
40 mM 0.1059 mL 0.5293 mL 1.0587 mL 2.6467 mL
50 mM 0.0847 mL 0.4235 mL 0.8470 mL 2.1174 mL
60 mM 0.0706 mL 0.3529 mL 0.7058 mL 1.7645 mL
80 mM 0.0529 mL 0.2647 mL 0.5293 mL 1.3234 mL
100 mM 0.0423 mL 0.2117 mL 0.4235 mL 1.0587 mL
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Isosorbide dinitrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isosorbide dinitrate87-33-2ISDNNO SynthaseNitric oxide synthasesNOSmyocardialinfarctionNO,reperfusioninjuryInhibitorinhibitorinhibit

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