IWP-3

Cat. No.: HY-100536 Purity: 98.23%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

IWP-3 is an potent inhibitor of Wnt production with an IC₅₀ of 40 nM. IWP-3 inhibits Porcupine (Porcn) function thereby blocking palmitoylation of Wnt proteins. IWP-3 inhibits CK1γ3 and CK1ε only moderately and does not inhibit CK1α.

For research use only. We do not sell to patients.

IWP-3 Chemical Structure

CAS No. : 687561-60-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 117	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 117	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CAPAN-1	EC₅₀	> 10 μM Compound: IWP-3	Antiproliferative activity against human Capan1 cells after 48 hrs by MTT assay Antiproliferative activity against human Capan1 cells after 48 hrs by MTT assay	[PMID: 29630366]
HEK293	EC₅₀	3.5 μM Compound: IWP-3	Antiproliferative activity against human HEK293 cells after 48 hrs by MTT assay Antiproliferative activity against human HEK293 cells after 48 hrs by MTT assay	[PMID: 29630366]
HEK-293T	IC₅₀	538 nM Compound: 3, IWP-3	Inhibition of beta-casein-dependent canonical Wnt3 pathway in human HEK293T cells by luciferase reporter gene assay Inhibition of beta-casein-dependent canonical Wnt3 pathway in human HEK293T cells by luciferase reporter gene assay	[PMID: 22191557]
HT-29	EC₅₀	> 10 μM Compound: IWP-3	Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 29630366]
MIA PaCa-2	EC₅₀	5.34 μM Compound: IWP-3	Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by MTT assay Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by MTT assay	[PMID: 29630366]
PANC-1	EC₅₀	4.87 μM Compound: IWP-3	Antiproliferative activity against human PANC1 cells after 48 hrs by MTT assay Antiproliferative activity against human PANC1 cells after 48 hrs by MTT assay	[PMID: 29630366]
SW-620	EC₅₀	8.62 μM Compound: IWP-3	Antiproliferative activity against human SW620 cells after 48 hrs by MTT assay Antiproliferative activity against human SW620 cells after 48 hrs by MTT assay	[PMID: 29630366]

Molecular Weight

484.59

Formula

C₂₂H₁₇FN₄O₂S₃

CAS No.

687561-60-0

Appearance

Solid

Color

White to light yellow

SMILES

O=C(NC1=NC2=CC=C(C)C=C2S1)CSC3=NC(CCS4)=C4C(N3C5=CC=C(F)C=C5)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (34.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0636 mL	10.3180 mL	20.6360 mL
5 mM	0.4127 mL	2.0636 mL	4.1272 mL
10 mM	0.2064 mL	1.0318 mL	2.0636 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.23%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (250 KB) HNMR (270 KB) RP-HPLC (231 KB) MS (197 KB)

Handling Instructions (2659 KB)

References

[1]. Baozhi Chen, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol. 2009 Feb;5(2):100-7. [Content Brief]

[2]. Balbina García-Reyes, et al. Discovery of Inhibitor of Wnt Production 2 (IWP-2) and Related Compounds As Selective ATP-Competitive Inhibitors of Casein Kinase 1 (CK1) δ/ε. J Med Chem. 2018 May 10;61(9):4087-4102. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0636 mL	10.3180 mL	20.6360 mL	51.5900 mL
	5 mM	0.4127 mL	2.0636 mL	4.1272 mL	10.3180 mL
	10 mM	0.2064 mL	1.0318 mL	2.0636 mL	5.1590 mL
	15 mM	0.1376 mL	0.6879 mL	1.3757 mL	3.4393 mL
	20 mM	0.1032 mL	0.5159 mL	1.0318 mL	2.5795 mL
	25 mM	0.0825 mL	0.4127 mL	0.8254 mL	2.0636 mL
	30 mM	0.0688 mL	0.3439 mL	0.6879 mL	1.7197 mL

IWP-3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IWP-3687561-60-0IWP3IWP 3WntPorcupinePorcnpalmitoylationCK1γ3CK1εCK1αInhibitorinhibitorinhibit

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IWP-3

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IWP-3 Related Antibodies

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Complete Stock Solution Preparation Table

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