Jolkinolide B

Name: Jolkinolide B
Brand: MedChemExpress
SKU: HY-N0732
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0732 Purity: 99.12%: FAQs
Data Sheet SDS COA Handling Instructions

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Jolkinolide B, a bioactive diterpene isolated from the roots of Euphorbia fischeriana Steud, is known to induce apoptosis in cancer cells.

For research use only. We do not sell to patients.

Jolkinolide B Chemical Structure

CAS No. : 37905-08-1

Size	Price	Stock	Quantity
5 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Jolkinolide B:

Jolkinolide B (Standard) Get quote

1 Publications Citing Use of MCE Jolkinolide B

•PLoS One. 2024 Apr 17;19(4):e0299920. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

Jolkinolide B, a bioactive diterpene isolated from the roots of Euphorbia fischeriana Steud, is known to induce apoptosis in cancer cells^[1].

IC₅₀ & Target

IC50: apoptosis^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	5.09 μg/mL Compound: 8	Cytotoxicity against human A2780 cells by MTT assay Cytotoxicity against human A2780 cells by MTT assay	[PMID: 18205316]
A549	IC₅₀	28.238 μM Compound: Compound 4	Cytotoxicity against human A549 cells assessed as cell growth inhibition by MTT assay Cytotoxicity against human A549 cells assessed as cell growth inhibition by MTT assay	[PMID: 27913179]
A549	IC₅₀	6.1 μg/mL Compound: 8	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 18205316]
Bel-7402	IC₅₀	5.95 μg/mL Compound: 8	Cytotoxicity against human Bel 7402 cells by MTT assay Cytotoxicity against human Bel 7402 cells by MTT assay	[PMID: 18205316]
BGC-823	IC₅₀	5.81 μg/mL Compound: 8	Cytotoxicity against human BGC 823 cells by MTT assay Cytotoxicity against human BGC 823 cells by MTT assay	[PMID: 18205316]
HCT-15	IC₅₀	48 μM Compound: 6	Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay	[PMID: 33289552]
HCT-8	IC₅₀	6.88 μg/mL Compound: 8	Cytotoxicity against human HCT8 cells by MTT assay Cytotoxicity against human HCT8 cells by MTT assay	[PMID: 18205316]
Hep 3B2	IC₅₀	> 100 μM Compound: Compound 4	Cytotoxicity against human Hep3B cells assessed as cell growth inhibition by MTT assay Cytotoxicity against human Hep3B cells assessed as cell growth inhibition by MTT assay	[PMID: 27913179]
LNCaP C4-2B	IC₅₀	4.4 μM Compound: 6	Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay	[PMID: 33289552]
MCF7	IC₅₀	9.1 μM Compound: 6	Cytotoxicity against human MCF7 cells incubated for 48 hrs by sulforhodamine B staining based spectrophotometric assay Cytotoxicity against human MCF7 cells incubated for 48 hrs by sulforhodamine B staining based spectrophotometric assay	[PMID: 33289552]
MDA-MB-231	IC₅₀	31 μM Compound: 6	Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay	[PMID: 33289552]
RAW264.7	IC₅₀	0.88 μM Compound: 7	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 26615888]
RAW264.7	IC₅₀	4.9 μM Compound: 6	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess assay Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess assay	[PMID: 26702644]
UACC-62	IC₅₀	2.8 μM Compound: 6	Cytotoxicity against human UACC-62 cells incubated for 48 hrs by sulforhodamine B staining based spectrophotometric assay Cytotoxicity against human UACC-62 cells incubated for 48 hrs by sulforhodamine B staining based spectrophotometric assay	[PMID: 33289552]

Molecular Weight

330.42

Formula

C₂₀H₂₆O₄

CAS No.

37905-08-1

Appearance

Solid

Color

White to off-white

SMILES

C[C@]([C@]1([H])CC2)(CCCC1(C)C)[C@@]([C@@H]3[C@@]4(O5)O3)([H])[C@@]62[C@@H](C4=C(C)C5=O)O6

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 8.33 mg/mL (25.21 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0265 mL	15.1323 mL	30.2645 mL
5 mM	0.6053 mL	3.0265 mL	6.0529 mL
10 mM	0.3026 mL	1.5132 mL	3.0265 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (2.51 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 20% HP-β-CD/1% Tween-80 in PBS
Solubility: 3.33 mg/mL (10.08 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.12%

Select Batch:

Data Sheet (276 KB) SDS (392 KB)

COA (254 KB) HNMR (220 KB) RP-HPLC (407 KB)

Handling Instructions (2659 KB)

References

[1]. Wang, J., Zhou, Y., Bai, X. et al. Jolkinolide B from Euphorbia fischeriana Steud induces apoptosis in human leukemic U937 cells through PI3K/Akt and XIAP pathways. Mol Cells 32, 451–457 (2011). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0265 mL	15.1323 mL	30.2645 mL	75.6613 mL
	5 mM	0.6053 mL	3.0265 mL	6.0529 mL	15.1323 mL
	10 mM	0.3026 mL	1.5132 mL	3.0265 mL	7.5661 mL
	15 mM	0.2018 mL	1.0088 mL	2.0176 mL	5.0441 mL
	20 mM	0.1513 mL	0.7566 mL	1.5132 mL	3.7831 mL
	25 mM	0.1211 mL	0.6053 mL	1.2106 mL	3.0265 mL

Jolkinolide B Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Jolkinolide B37905-08-1ApoptosiscancerhumanleukemicU937cellsInhibitorinhibitorinhibit

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Jolkinolide B

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1 Publications Citing Use of MCE Jolkinolide B

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Complete Stock Solution Preparation Table

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