Kirenol

Name: Kirenol
Brand: MedChemExpress
SKU: HY-N0559
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0559 Purity: 99.34%: FAQs
Data Sheet SDS COA Handling Instructions

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Kirenol is isolated from Siegesbeckia orientalis with anti-inflammatory and analgesic activity.

For research use only. We do not sell to patients.

Kirenol Chemical Structure

CAS No. : 52659-56-0

Size	Price	Stock	Quantity
5 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 63	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
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200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Kirenol (Standard) Get quote

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Description

Kirenol is isolated from Siegesbeckia orientalis with anti-inflammatory and analgesic activity^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	> 100 μg/mL Compound: 5	Cytotoxicity against mouse B16 cells after 44 hrs by MTT assay Cytotoxicity against mouse B16 cells after 44 hrs by MTT assay	[PMID: 19928884]
HSC-T6	IC₅₀	> 100 μg/mL Compound: 5	Cytotoxicity against rat HSC-T6 cells after 44 hrs by MTT assay Cytotoxicity against rat HSC-T6 cells after 44 hrs by MTT assay	[PMID: 19928884]
MDA-MB-231	IC₅₀	> 10 μM Compound: 19	Antiinvasive activity in human MDA-MB-231 cells assessed as inhibition of EGF-induced cell invasion after 3.5 hrs Antiinvasive activity in human MDA-MB-231 cells assessed as inhibition of EGF-induced cell invasion after 3.5 hrs	[PMID: 28009521]
RAW264.7	IC₅₀	> 100 μM Compound: 19	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28009521]

Molecular Weight

338.48

Formula

C₂₀H₃₄O₄

CAS No.

52659-56-0

Appearance

Solid

Color

Off-white to light yellow

SMILES

C[C@]([C@@](CC1)([H])[C@]2(C)CO)(C[C@H](O)C2)[C@@]3([H])C1=C[C@]([C@@H](O)CO)(C)CC3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (295.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.1 mg/mL (0.30 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9544 mL	14.7719 mL	29.5437 mL
5 mM	0.5909 mL	2.9544 mL	5.9087 mL
10 mM	0.2954 mL	1.4772 mL	2.9544 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (9.60 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.25 mg/mL (9.60 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.25 mg/mL (9.60 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.34%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (250 KB) LCMS (114 KB)

Handling Instructions (2659 KB)

References

[1]. Jian-ping Wang, et al. Topical anti-inflammatory and analgesic activity of kirenol isolated from Siegesbeckia orientalis, Journal of Ethnopharmacology, Volume 137, Issue 3, 2011, Pages 1089-1094, ISSN 0378-8741. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9544 mL	14.7719 mL	29.5437 mL	73.8593 mL
	5 mM	0.5909 mL	2.9544 mL	5.9087 mL	14.7719 mL
	10 mM	0.2954 mL	1.4772 mL	2.9544 mL	7.3859 mL
	15 mM	0.1970 mL	0.9848 mL	1.9696 mL	4.9240 mL
	20 mM	0.1477 mL	0.7386 mL	1.4772 mL	3.6930 mL
	25 mM	0.1182 mL	0.5909 mL	1.1817 mL	2.9544 mL
	30 mM	0.0985 mL	0.4924 mL	0.9848 mL	2.4620 mL
	40 mM	0.0739 mL	0.3693 mL	0.7386 mL	1.8465 mL
	50 mM	0.0591 mL	0.2954 mL	0.5909 mL	1.4772 mL
	60 mM	0.0492 mL	0.2462 mL	0.4924 mL	1.2310 mL
	80 mM	0.0369 mL	0.1846 mL	0.3693 mL	0.9232 mL
	100 mM	0.0295 mL	0.1477 mL	0.2954 mL	0.7386 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Kirenol Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kirenol52659-56-0OthersSiegesbeckiaorientalisanti-inflammatoryanalgesicInhibitorinhibitorinhibit

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Kirenol

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Other Forms of Kirenol:

Kirenol Related Antibodies

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Complete Stock Solution Preparation Table

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