2. Immunology/Inflammation
  3. NO Synthase
  4. L-NIL hydrochloride

L-NIL hydrochloride is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for mouse inducible NOS.

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L-NIL hydrochloride Chemical Structure

L-NIL hydrochloride Chemical Structure

CAS No. : 150403-89-7

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L-NIL hydrochloride Related Antibodies

Top Publications Citing Use of Products

    L-NIL hydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 5 March 2022.

    Inhibiting iNOS with L-NIL (10 μM) blunted the adverse effect of iNOS expression in cardiomyocytes.

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    nNOS iNOS eNOS

    • Biological Activity

    • Purity & Documentation

    • References

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    Description

    L-NIL hydrochloride is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for mouse inducible NOS[1][2][3].

    IC50 & Target

    IC50: 3.3 μM (mouse inducible NO synthase), 92 μM (rat brain constitutive NO synthase)[1].
    In Vitro

    L-NIL produces a concentration-dependent inhibition of both the mouse inducible NOS and the rat brain constitutive NOS (rcNOS) and is considerably more potent for mouse inducible NOS. The IC50 values for L-NIL with mouse inducible NOS and rcNOS are 3.3 and 92 pM, respectively, indicating that L-NIL is 28-fold more selective for mouse inducible NOS. In addition, L-NIL has approximately 6-fold greater potency for mouse inducible NOS than either L-NMA or L-NNA[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-NIL hydrochloride Related Antibodies
    In Vivo

    L-NIL (10 and 30 mg/kg, IP) prevents the inflammation, oxidative stress and autophagy induced by renal IR in mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Balb/c (20-25 g)[1].
    Dosage: 10 and 30 mg/kg.
    Administration: Intraperitoneally at the end of CLP and at 6 h after sepsis induction.
    Result: Led to a negligible increase in plasma NGAL compared to sham mice.
    Led to a significant decrease in both TLR4 and IL1βprotein contents and clusterin transcript.
    Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle.
    Promoted a decrease in AR protein expression, as compared with animals treated with vehicle.
    Molecular Weight

    223.70

    Formula

    C8H18ClN3O2
    CAS No.
    SMILES

    OC([C@@H](N)CCCCNC(C)=N)=O.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (447.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.4703 mL 22.3514 mL 44.7027 mL
    5 mM 0.8941 mL 4.4703 mL 8.9405 mL
    View the Complete Stock Solution Preparation Table

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (11.18 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 2.5 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (11.18 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 2.5 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.4703 mL 22.3514 mL 44.7027 mL 111.7568 mL
    5 mM 0.8941 mL 4.4703 mL 8.9405 mL 22.3514 mL
    10 mM 0.4470 mL 2.2351 mL 4.4703 mL 11.1757 mL
    15 mM 0.2980 mL 1.4901 mL 2.9802 mL 7.4505 mL
    20 mM 0.2235 mL 1.1176 mL 2.2351 mL 5.5878 mL
    25 mM 0.1788 mL 0.8941 mL 1.7881 mL 4.4703 mL
    30 mM 0.1490 mL 0.7450 mL 1.4901 mL 3.7252 mL
    40 mM 0.1118 mL 0.5588 mL 1.1176 mL 2.7939 mL
    50 mM 0.0894 mL 0.4470 mL 0.8941 mL 2.2351 mL
    60 mM 0.0745 mL 0.3725 mL 0.7450 mL 1.8626 mL
    80 mM 0.0559 mL 0.2794 mL 0.5588 mL 1.3970 mL
    100 mM 0.0447 mL 0.2235 mL 0.4470 mL 1.1176 mL
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    L-NIL hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-NIL150403-89-7NO SynthaseNitric oxide synthasesNOSiNOSNFAT5 TLR-4GSTclusterinIL1βInhibitorinhibitorinhibit

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