L-NIL

Name: L-NIL
Brand: MedChemExpress
SKU: HY-12116
Rating: 5.0 (8 reviews)

Cat. No.: HY-12116 Purity: 99.82%: FAQs
Data Sheet SDS COA Handling Instructions

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L-NIL is an inducible NO synthase inhibitor, with an IC₅₀ of 3.3 μM for miNOS.

For research use only. We do not sell to patients.

L-NIL Chemical Structure

CAS No. : 53774-63-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 99	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 99	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of L-NIL:

L-NIL hydrochloride Get quote
L-NIL dihydrochloride Get quote

7 Publications Citing Use of MCE L-NIL

•Exp Eye Res. 2024 Apr 5:243:109886. [Abstract]

: L-NIL purchased from MedChemExpress. Usage Cited in: J Adv Res. 5 March 2022.; Inhibiting iNOS with L-NIL (10 μM) blunted the adverse effect of iNOS expression in cardiomyocytes.

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

L-NIL is an inducible NO synthase inhibitor, with an IC₅₀ of 3.3 μM for miNOS^[1]^[2]^[3].

IC₅₀ & Target

iNOS

Cellular Effect

Cell Line	Type	Value	Description	References
DLD-1	IC₅₀	0.13 μM Compound: 3	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells	[PMID: 11327580]
DLD-1	IC₅₀	2.8 μM Compound: 3	Inhibitory activity against synthesis of inducible nitric oxide synthase by DLD-1 cells Inhibitory activity against synthesis of inducible nitric oxide synthase by DLD-1 cells	[PMID: 12620067]
DLD-1	IC₅₀	280 nM Compound: 1	Inhibition of human iNOS expressed in human DLD1 cells after 1 hr Inhibition of human iNOS expressed in human DLD1 cells after 1 hr	[PMID: 18024030]
DLD-1	IC₅₀	31000 nM Compound: 1	Inhibition of cytokine-induced iNOS activity in human DLD1 cells after 24 hrs Inhibition of cytokine-induced iNOS activity in human DLD1 cells after 24 hrs	[PMID: 18024030]
HUVEC	IC₅₀	2.4 μM Compound: 3	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells	[PMID: 11327580]
RAW264.7	IC₅₀	> 200 μM Compound: L-NIL	Cytotoxicity against LPS-stimulated mouse RAW264.7 cells by Griess method Cytotoxicity against LPS-stimulated mouse RAW264.7 cells by Griess method	[PMID: 14510614]
RAW264.7	IC₅₀	1.55 μM Compound: NIL	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pretreated for 1 hr followed by LPS-stimulation and measured after 24 hrs by Griess assay Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pretreated for 1 hr followed by LPS-stimulation and measured after 24 hrs by Griess assay	[PMID: 29932657]
RAW264.7	IC₅₀	15.8 μM Compound: L-NIL	Inhibition of LPS-stimulated nitric oxide production in rat RAW264.7 cells after 24 hrs by Griess assay Inhibition of LPS-stimulated nitric oxide production in rat RAW264.7 cells after 24 hrs by Griess assay	[PMID: 29799195]
RAW264.7	IC₅₀	15.8 μM Compound: L-NIL	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25666824]
RAW264.7	IC₅₀	18.6 μM Compound: L-NIL	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method	[PMID: 14510614]
RAW264.7	IC₅₀	20.14 μM Compound: L-NIL	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay	[PMID: 24360561]
RAW264.7	IC₅₀	28.29 μM Compound: L-NIL	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay	[PMID: 29102229]
RAW264.7	IC₅₀	7 μM Compound: L-Nil	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation and measured after 24 hrs by Griess reagent based colorimetric method Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation and measured after 24 hrs by Griess reagent based colorimetric method	[PMID: 30724564]
RAW264.7	IC₅₀	7.5 μM Compound: L-NIL	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay	[PMID: 27588583]
Sf9	IC₅₀	2500 nM Compound: 1	Inhibition of human eNOS expressed in insect SF9 cells after 1 hr Inhibition of human eNOS expressed in insect SF9 cells after 1 hr	[PMID: 18024030]
Sf9	IC₅₀	6000 nM Compound: 1	Inhibition of human nNOS expressed in insect SF9 cells after 1 hr Inhibition of human nNOS expressed in insect SF9 cells after 1 hr	[PMID: 18024030]

In Vitro

L-NIL produces a concentration-dependent inhibition of both the mouse inducible NOS (miNOS) and the rat brain constitutive NOS (rcNOS) and is considerably more potent for miNOS. The IC₅₀ values for L-NIL with miNOS and rcNOS are 3.3 and 92 pM, respectively, indicating that L-NIL is 28-fold more selective for miNOS. In addition, L-NIL has approximately 6-fold greater potency for miNOS than either L-NMA or L-NNA^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-NIL (10 and 30 mg/kg, IP) prevents the inflammation, oxidative stress and autophagy induced by renal IR in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult male Balb/c (20-25 g)^[1].
Dosage:	10 and 30 mg/kg.
Administration:	Intraperitoneally at the end of CLP and at 6 h after sepsis induction.
Result:	Led to a negligible increase in plasma NGAL compared to sham mice. Led to a significant decrease in both TLR4 and IL1β protein contents and clusterin transcript. Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle. Promoted a decrease in AR protein expression, as compared with animals treated with vehicle.

Molecular Weight

187.24

Formula

C₈H₁₇N₃O₂

CAS No.

53774-63-3

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCCNC(C)=N)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (267.04 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3407 mL	26.7037 mL	53.4074 mL
5 mM	1.0681 mL	5.3407 mL	10.6815 mL
10 mM	0.5341 mL	2.6704 mL	5.3407 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (534.07 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (242 KB) HNMR (321 KB) RP-HPLC (245 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Consuelo Pasten, et al. l-NIL prevents the ischemia and reperfusion injury involving TLR-4, GST, clusterin, and NFAT-5 in mice. Am J Physiol Renal Physiol. 2019 Apr 1;316(4):F624-F634. [Content Brief]

[2]. Sharon Angela Tanuseputero, et al. Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis. Mediators Inflamm. 2020 May 11;2020:3201635. [Content Brief]

[3]. Moore WM, et al. L-N6-(1-iminoethyl)lysine: a selective inhibitor of inducible nitric oxide synthase. J Med Chem. 1994 Nov 11;37(23):3886-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.3407 mL	26.7037 mL	53.4074 mL	133.5185 mL
	5 mM	1.0681 mL	5.3407 mL	10.6815 mL	26.7037 mL
	10 mM	0.5341 mL	2.6704 mL	5.3407 mL	13.3518 mL
	15 mM	0.3560 mL	1.7802 mL	3.5605 mL	8.9012 mL
	20 mM	0.2670 mL	1.3352 mL	2.6704 mL	6.6759 mL
	25 mM	0.2136 mL	1.0681 mL	2.1363 mL	5.3407 mL
	30 mM	0.1780 mL	0.8901 mL	1.7802 mL	4.4506 mL
	40 mM	0.1335 mL	0.6676 mL	1.3352 mL	3.3380 mL
	50 mM	0.1068 mL	0.5341 mL	1.0681 mL	2.6704 mL
	60 mM	0.0890 mL	0.4451 mL	0.8901 mL	2.2253 mL
	80 mM	0.0668 mL	0.3338 mL	0.6676 mL	1.6690 mL
	100 mM	0.0534 mL	0.2670 mL	0.5341 mL	1.3352 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-NIL Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-NIL53774-63-3NO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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L-NIL

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Other Forms of L-NIL:

L-NIL Related Antibodies

7 Publications Citing Use of MCE L-NIL

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NO Synthase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

L-NIL Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

L-NIL Related Classifications

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