2. Immunology/Inflammation
  3. NO Synthase
  4. L-NIL

L-NIL is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for miNOS.

For research use only. We do not sell to patients.

L-NIL Chemical Structure

L-NIL Chemical Structure

CAS No. : 53774-63-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 99 In-stock
Solution
10 mM * 1 mL in Water USD 99 In-stock
Solid
50 mg USD 90 In-stock
100 mg USD 140 In-stock
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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of L-NIL:

L-NIL Related Antibodies

Top Publications Citing Use of Products

    L-NIL purchased from MedChemExpress. Usage Cited in: J Adv Res. 5 March 2022.

    Inhibiting iNOS with L-NIL (10 μM) blunted the adverse effect of iNOS expression in cardiomyocytes.

    View All NO Synthase Isoform Specific Products:

    View All Isoforms
    nNOS iNOS eNOS

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-NIL is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for miNOS[1][2][3].

    IC50 & Target

    iNOS

     

    In Vitro

    L-NIL produces a concentration-dependent inhibition of both the mouse inducible NOS (miNOS) and the rat brain constitutive NOS (rcNOS) and is considerably more potent for miNOS. The IC50 values for L-NIL with miNOS and rcNOS are 3.3 and 92 pM, respectively, indicating that L-NIL is 28-fold more selective for miNOS. In addition, L-NIL has approximately 6-fold greater potency for miNOS than either L-NMA or L-NNA[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-NIL Related Antibodies
    In Vivo

    L-NIL (10 and 30 mg/kg, IP) prevents the inflammation, oxidative stress and autophagy induced by renal IR in mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Balb/c (20-25 g)[1].
    Dosage: 10 and 30 mg/kg.
    Administration: Intraperitoneally at the end of CLP and at 6 h after sepsis induction.
    Result: Led to a negligible increase in plasma NGAL compared to sham mice.
    Led to a significant decrease in both TLR4 and IL1β protein contents and clusterin transcript.
    Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle.
    Promoted a decrease in AR protein expression, as compared with animals treated with vehicle.
    Molecular Weight

    187.24

    Formula

    C8H17N3O2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@@H](CCCCNC(C)=N)C(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (267.04 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.3407 mL 26.7037 mL 53.4074 mL
    5 mM 1.0681 mL 5.3407 mL 10.6815 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (534.07 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.82%

    COA (242 KB) HNMR (321 KB) RP-HPLC (245 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.3407 mL 26.7037 mL 53.4074 mL 133.5185 mL
    5 mM 1.0681 mL 5.3407 mL 10.6815 mL 26.7037 mL
    10 mM 0.5341 mL 2.6704 mL 5.3407 mL 13.3518 mL
    15 mM 0.3560 mL 1.7802 mL 3.5605 mL 8.9012 mL
    20 mM 0.2670 mL 1.3352 mL 2.6704 mL 6.6759 mL
    25 mM 0.2136 mL 1.0681 mL 2.1363 mL 5.3407 mL
    30 mM 0.1780 mL 0.8901 mL 1.7802 mL 4.4506 mL
    40 mM 0.1335 mL 0.6676 mL 1.3352 mL 3.3380 mL
    50 mM 0.1068 mL 0.5341 mL 1.0681 mL 2.6704 mL
    60 mM 0.0890 mL 0.4451 mL 0.8901 mL 2.2253 mL
    80 mM 0.0668 mL 0.3338 mL 0.6676 mL 1.6690 mL
    100 mM 0.0534 mL 0.2670 mL 0.5341 mL 1.3352 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-NIL Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-NIL53774-63-3NO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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