Lawsone

Name: Lawsone
Brand: MedChemExpress
SKU: HY-N2493
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2493 Purity: 99.36%: FAQs
Data Sheet SDS COA Handling Instructions

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Lawsone is an orally active naphthoquinone dye that can be isolated from the leaves of Lawsonia inermis. Lawsone can induce apoptosis. Lawsone has antibacterial, antitumor and antioxidant activities. Lawsone can be used in anti-tumor drug research.

For research use only. We do not sell to patients.

Lawsone Chemical Structure

CAS No. : 83-72-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
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5 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Lawsone:

Lawsone-d₄ Get quote

1 Publications Citing Use of MCE Lawsone

•Int J Biol Macromol. 2021 Apr 24.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	58 μM Compound: 17	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 24374273]
HeLa	IC₅₀	> 100 μM Compound: 1c	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24913712]
Hepatocyte	CC₅₀	> 500 μM Compound: Lawsone	Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]
HFF-1	IC₅₀	> 50 μM Compound: 7	Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34438124]
Hs 683	IC₅₀	31 μM Compound: 17	Growth inhibition of human Hs683 cells by MTT assay Growth inhibition of human Hs683 cells by MTT assay	[PMID: 24374273]
LoVo	IC₅₀	> 100 μM Compound: 17	Growth inhibition of human LoVo cells by MTT assay Growth inhibition of human LoVo cells by MTT assay	[PMID: 24374273]
MCF7	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against ER-positive human MCF7 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against ER-positive human MCF7 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay	[PMID: 31810778]
MDA-MB-231	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay	[PMID: 31810778]
PC-3	IC₅₀	> 100 μM Compound: 17	Growth inhibition of human PC3 cells by MTT assay Growth inhibition of human PC3 cells by MTT assay	[PMID: 24374273]
SAS	IC₅₀	> 100 μM Compound: 1c	Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24913712]
SK-BR-3	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against HER2-positive human SKBR3 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against HER2-positive human SKBR3 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay	[PMID: 31810778]
SK-MEL-28	IC₅₀	> 100 μM Compound: 17	Growth inhibition of human SK-MEL-28 cells by MTT assay Growth inhibition of human SK-MEL-28 cells by MTT assay	[PMID: 24374273]
U-373MG ATCC	IC₅₀	> 100 μM Compound: 17	Growth inhibition of human U373 cells by MTT assay Growth inhibition of human U373 cells by MTT assay	[PMID: 24374273]
Vero	CC₅₀	213.74 μM Compound: Lawsone	Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]

In Vitro

Lawsone (125, 250, 500 μM, 18 h) inhibits the growth of Escherichia coli in a dose-dependent manner^[3].
Lawsone (0.5, 1, 1.5, 2 mg/mL, 48 h) inhibits cell proliferation in human DLD-1 cells by reducing NF-κB activity, resulting in inhibition of the expression levels of cyclin B1 and cdk1^[4].
Lawsone (10, 20, 40, 80, 160 mg/mL, 24 h) has an anti-proliferation effect in SKOV-3 ovarian cancer cells and induces apoptosis by inhibiting Bcl-2^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[4]

Cell Line:	DLD-1, MRC-5
Concentration:	0.5, 1, 1.5, 2 mg/mL
Incubation Time:	24-96 h
Result:	Demonstrated significant suppression at concentration 2 mg/ml on DLD-1 cells and exhibited no suppression on normal fibroblast MRC-5 cells.

Western Blot Analysis^[4]

Cell Line:	DLD-1
Concentration:	0.5, 1, 1.5, 2 mg/mL
Incubation Time:	48 h
Result:	Decreased the level of both proteins cyclin B1 and cdk1. Exhibited a dose-dependent effect on translocation of p65 of cytosol to the nucleus.

In Vivo

Lawsone (62.5, 125, 250, 500, 750 μmol/kg/day, orally) induces hemolytic reactions associated with oxidative damage of red blood cells in rats^[2].
Lawsone (200 mg/mL, orally, for 8 weeks) has a significant inhibitory effect on colon cancer in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female rat model (10-11- week-old)^[2]
Dosage:	62.5, 125, 250, 500, 750 μmol/kg/day
Administration:	p.o.
Result:	Increased the levels of ALT, AST, GLDH, LDH, HBDH, urea and creatinine at a dose of 500 or 750 μmol/kg/day.

Animal Model:	Rat model of colon cancer^[4]
Dosage:	200 mg/mL
Administration:	p.o.
Result:	Reduced the tumor diameters and decreased multiplicity of these adenomas. Decreased the PCNA staining index

Molecular Weight

174.16

Formula

C₁₀H₆O₃

CAS No.

83-72-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O)=CC(C2=C1C=CC=C2)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (574.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7420 mL	28.7101 mL	57.4201 mL
5 mM	1.1484 mL	5.7420 mL	11.4840 mL
10 mM	0.5742 mL	2.8710 mL	5.7420 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.36%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (250 KB) HNMR (127 KB) RP-HPLC (221 KB)

Handling Instructions (2659 KB)

References

[1]. Tripathi RD, et al. A fungitoxic principle from the leaves of lawsonia inermis lam. Experientia. 1978 Jan 15;34(1):51-2. [Content Brief]

[2]. Munday R, et al. Haemolytic activity and nephrotoxicity of 2-hydroxy-1,4-naphthoquinone in rats. J Appl Toxicol. 1991 Apr;11(2):85-90. [Content Brief]

[3]. Sauriasari R, et al. Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli. Toxicology. 2007 Jun 3;235(1-2):103-11. [Content Brief]

[4]. Wang SB, et al. Lawsone suppresses azoxymethane mediated colon cancer in rats and reduces proliferation of DLD-1 cells via NF-κB pathway. Biomed Pharmacother. 2017 May;89:152-161. [Content Brief]

[5]. Li P, et al. Lawsone inhibits cell growth and improves the efficacy of cisplatin in SKOV-3 ovarian cancer cell lines. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(5): 8-17.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.7420 mL	28.7101 mL	57.4201 mL	143.5503 mL
	5 mM	1.1484 mL	5.7420 mL	11.4840 mL	28.7101 mL
	10 mM	0.5742 mL	2.8710 mL	5.7420 mL	14.3550 mL
	15 mM	0.3828 mL	1.9140 mL	3.8280 mL	9.5700 mL
	20 mM	0.2871 mL	1.4355 mL	2.8710 mL	7.1775 mL
	25 mM	0.2297 mL	1.1484 mL	2.2968 mL	5.7420 mL
	30 mM	0.1914 mL	0.9570 mL	1.9140 mL	4.7850 mL
	40 mM	0.1436 mL	0.7178 mL	1.4355 mL	3.5888 mL
	50 mM	0.1148 mL	0.5742 mL	1.1484 mL	2.8710 mL
	60 mM	0.0957 mL	0.4785 mL	0.9570 mL	2.3925 mL
	80 mM	0.0718 mL	0.3589 mL	0.7178 mL	1.7944 mL
	100 mM	0.0574 mL	0.2871 mL	0.5742 mL	1.4355 mL

Lawsone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lawsone83-72-7FungalApoptosisAntitumorDLD-1SKOV-3Colon cancer rat modelInhibitorinhibitorinhibit

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Lawsone

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Other Forms of Lawsone:

Lawsone Related Antibodies

1 Publications Citing Use of MCE Lawsone

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•Related Screening Libraries:

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References

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Complete Stock Solution Preparation Table

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