2. Apoptosis Immunology/Inflammation
  3. Apoptosis SOD
  4. LCS-1

LCS-1 is a superoxide dismutase 1 (SOD1) inhibitor. LCS-1 inhibits SOD1 activity with an IC50 value of 1.07 μM. LCS-1 induces the early- and late-stage apoptosis of multiple myeloma (MM.1S) cells.

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LCS-1 Chemical Structure

LCS-1 Chemical Structure

CAS No. : 41931-13-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 90 In-stock
25 mg USD 200 In-stock
50 mg USD 350 In-stock
100 mg USD 650 In-stock
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Based on 1 publication(s) in Google Scholar

LCS-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE LCS-1

  • Biological Activity

  • Purity & Documentation

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Description

LCS-1 is a superoxide dismutase 1 (SOD1) inhibitor. LCS-1 inhibits SOD1 activity with an IC50 value of 1.07 μM. LCS-1 induces the early- and late-stage apoptosis of multiple myeloma (MM.1S) cells[1][2][3].

IC50 & Target[2]

SOD1

1.07 μM (IC50)

In Vitro

LCS-1 (1-10000 nM; 24 hours) has selective cytotoxicity towards bloom syndrome gene product (BLM) -proficient and BLM-deficient HCT116 cells[1].
LCS-1 shows growth inhibitory effect on 10/27 adenocarcinoma cell lines (median IC50=0.20 μM; such as H23, H2347, HCC827 cell lines) and normal human bronchial epithelial (NHBE) cells (IC50=2.66 μM)[2].
LCS-1 (0, 1.25, 2 μM; 4 h) in a concentration-dependent manner triggers significant inhibition of SOD1 enzymatic activity in multiple myeloma (MM) cells[3].
LCS-1 (0, 1.25, 2.5, 5 μM; 48 h) in a dose-dependent manner reduces the viability of various MM cell lines, including MM.1R (Dexamethasone-resistant), Dox40 (Doxorubicin-resistant), or LR5 (Melphalan-resistant) cell lines[3].
LCS-1 (48 h) has IC50 values of 2.5 and 4.6 μM for cell viability of ANBL6-WT (Bortezomib-sensitive) and ANBL6-BR (Bortezomib- resistant) cells, respectively[3].
LCS-1 (1.25 μM; 16 h) induces a significant increase in ROS levels and O2 levels in MM.1S cells[3].
LCS-1 (1.25 μM; 16 h) shows a significant decrease in GSH/GSSG ratio in MM.1S cells[3].
LCS-1 (1.25 μM; 24h) induces the release of mitochondrial cytochrome-c into the cytosol, and enriches the proteins (HSP60/CLPP) mediating mtUPR signaling in MM.1S cells[3].
LCS-1-induced O2 (1.25 μM; 5 h) triggers a marked decrease in both RP2CP and RP1CP forms of 26S proteasomes[3].
LCS-1 (2 μM; 16 h) induces the early- and late-stage apoptosis of MM.1S cells[3].
LCS-1 (0, 0.5, 1, 1.5, 2 μM) upregulates p53/p21 signaling, as well as downregulates survival pathway proteins MCL-1, BclxL, or c-Myc in MM.1S cells[3].
LCS-1 (0, 4, 8, 16, 24 h; 2 μM) shows a rapid and robust induction of mitochondrial unfolded protein response (UPR) proteins (BIP, PERK, phosphorylated eIF2α, or a lectin protein calnexin) in MM.1S and ANBL6-BR cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LCS-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: BLM-proficient and BLM-deficient HCT116 cells
Concentration: 1-10000 nM
Incubation Time: 24 hours
Result: Had IC50 values of 1462 nM and 24.92 nM for the viability of BLM-proficient and BLM-deficient HCT116 cells, respectively.

Western Blot Analysis[3]

Cell Line: MM.1S and ANBL6-BR cells
Concentration: 2 μM
Incubation Time: 16 hours
Result: Decreased the expression of cell-cycle regulatory proteins (cyclin-B1, CDC25C, and CDC2).

Western Blot Analysis[3]

Cell Line: MM.1S cells
Concentration: 0, 0.5, 1, 1.5, 2 μM
Incubation Time:
Result: Upregulated p53/p21 signaling, as well as downregulated survival pathway proteins MCL-1, BclxL, or c-Myc.

Western Blot Analysis[3]

Cell Line: MM.1S cells
Concentration: 2 μM
Incubation Time: 0, 4, 8, 16, 24 hours
Result: Showed a rapid and robust induction of UPR proteins (BIP, PERK, phosphorylated eIF2α, or a lectin protein calnexin).
In Vivo

LCS-1 (20 mg/kg; i.p. every other day for 14 days) inhibits MM growth and prolongs host survival in MM.1S-bearing mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-week-old female CB17 SCID mice (MM.1S tumors volume=100 mm3)[3]
Dosage: 20 mg/kg (diluted in saline)
Administration: Intraperitoneal injections; treated on an every other day schedule for 14 days
Result: Inhibited MM growth and prolongs host survival.
Molecular Weight

255.10

Formula

C11H8Cl2N2O
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C1C(Cl)=C(Cl)C=NN1C2=CC=CC(C)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (196.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9200 mL 19.6002 mL 39.2003 mL
5 mM 0.7840 mL 3.9200 mL 7.8401 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (9.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.59%

SDS (420 KB)

COA (248 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9200 mL 19.6002 mL 39.2003 mL 98.0008 mL
5 mM 0.7840 mL 3.9200 mL 7.8401 mL 19.6002 mL
10 mM 0.3920 mL 1.9600 mL 3.9200 mL 9.8001 mL
15 mM 0.2613 mL 1.3067 mL 2.6134 mL 6.5334 mL
20 mM 0.1960 mL 0.9800 mL 1.9600 mL 4.9000 mL
25 mM 0.1568 mL 0.7840 mL 1.5680 mL 3.9200 mL
30 mM 0.1307 mL 0.6533 mL 1.3067 mL 3.2667 mL
40 mM 0.0980 mL 0.4900 mL 0.9800 mL 2.4500 mL
50 mM 0.0784 mL 0.3920 mL 0.7840 mL 1.9600 mL
60 mM 0.0653 mL 0.3267 mL 0.6533 mL 1.6333 mL
80 mM 0.0490 mL 0.2450 mL 0.4900 mL 1.2250 mL
100 mM 0.0392 mL 0.1960 mL 0.3920 mL 0.9800 mL
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LCS-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LCS-141931-13-9LCS1LCS 1ApoptosisSODSuperoxide Dismutasecancermultiple myeloma cellsadenocarcinoma cellsDexamethasone-resistantp53/p21 signalingmitochondrial unfolded protein responseInhibitorinhibitorinhibit

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