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  5. Liquiritin apioside

Liquiritin apioside is an orally active inhibitor of TRPV1 receptor. Liquiritin apioside selectively inhibits laryngeal chemoreflex (LCR) and has no significant effect on mechanoreflex (LMR). Liquiritin apioside inhibits LCR by inhibiting reactive oxygen species (ROS) and NADPH oxidase, weakening the interaction between ROS and TRPV1. Liquiritin apioside can be used in the research of respiratory-related diseases.

For research use only. We do not sell to patients.

Liquiritin apioside Chemical Structure

Liquiritin apioside Chemical Structure

CAS No. : 74639-14-8

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Based on 1 publication(s) in Google Scholar

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  • Purity & Documentation

  • References

  • Customer Review

Description

Liquiritin apioside is an orally active inhibitor of TRPV1 receptor. Liquiritin apioside selectively inhibits laryngeal chemoreflex (LCR) and has no significant effect on mechanoreflex (LMR). Liquiritin apioside inhibits LCR by inhibiting reactive oxygen species (ROS) and NADPH oxidase, weakening the interaction between ROS and TRPV1. Liquiritin apioside can be used in the research of respiratory-related diseases[1][2].

In Vitro

Superior laryngeal nerve (SLN) related electrophysiological experiments: Liquiritin apioside (0.1 mM; overnight) can significantly change the electrophysiological kinetics of laryngeal C neuron currents induced by 1.5 μM Capsaicin (HY-10448P),reducing the current amplitude, increasing the rise time and prolonging the decay time[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Laryngeal C-neurons (isolated from nodose/jugular ganglia of rat pups)
Concentration: 0.1 mM
Incubation Time: Overnight; with 1.5 μM Capsaicin
Result: Inhibited the CAP-induced currents in laryngeal C-neurons.
Reduced the current amplitudes, increased the rise times, and prolonged the decay times. Of the eighteen laryngeal C-neurons tested, three neurons (16%) were not CAP-responsive.
In Vivo

Liquiritin apioside (10 μg/μL, intralaryngeal or perineural administration; single dose; 10 min, 20 min) significantly attenuates the apnea, hypertension, and bradycardia responses to the laryngeal chemoreflex (LCR) induced by Capsaicin (HY-10448),hydrochloric acid (HCl),and distilled water (DW) in rat pups, but has no significant effect on the laryngeal mechanoreflex (LMR) induced by air pulse (AP) [1].
Liquiritin apioside (0.01 g/kg, 0.2 g/kg; po; once daily for 10 days) ameliorates DSS-induced colitis in mice, and modulates the gut microbial diversity and composition[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Laryngeal reflexe study in Sprague-Dawley rat (250-350 g) pups[1]
Dosage: 10 μg/μL (diluted in saline)
Administration: Intralaryngeal perfusion for 10 minutes or peri-treatment for 20 minutes, single dose
Result: Significantly reduced the apneic responses to CAP, HCl, and DW, as well as attenuated the hypertension and bradycardia responses to these stimuli.
Did not change the cardiorespiratory responses to AP.
Peri-SLN treatment with Liquiritin apioside also suppressed the apnea, hypertension, and bradycardia responses to CAP, HCl, and DW, similar to the effect of intralaryngeal treatment.
Animal Model: DSS-induced colitis model in C57BL/6 mice (male, 18–22 g, 8–10 weeks)[2]
Dosage: 0.01 g/kg, 0.02 g/kg (diluted in saline)
Administration: Oral gavaged for 10 days, once daily
Result: Exhibited the same pathological changes as that of the ABX (DSS) group mice, specifically, comparable weight loss, DAI score, colon length, and histology score.
Molecular Weight

550.51

Formula

C26H30O13

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]([C@H]1O[C@]2([H])OC[C@](O)(CO)[C@H]2O)[C@H](O)[C@@H](CO)O[C@H]1OC(C=C3)=CC=C3[C@@H]4CC(C5=CC=C(O)C=C5O4)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (181.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8165 mL 9.0825 mL 18.1650 mL
5 mM 0.3633 mL 1.8165 mL 3.6330 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.60%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8165 mL 9.0825 mL 18.1650 mL 45.4124 mL
5 mM 0.3633 mL 1.8165 mL 3.6330 mL 9.0825 mL
10 mM 0.1816 mL 0.9082 mL 1.8165 mL 4.5412 mL
15 mM 0.1211 mL 0.6055 mL 1.2110 mL 3.0275 mL
20 mM 0.0908 mL 0.4541 mL 0.9082 mL 2.2706 mL
25 mM 0.0727 mL 0.3633 mL 0.7266 mL 1.8165 mL
30 mM 0.0605 mL 0.3027 mL 0.6055 mL 1.5137 mL
40 mM 0.0454 mL 0.2271 mL 0.4541 mL 1.1353 mL
50 mM 0.0363 mL 0.1816 mL 0.3633 mL 0.9082 mL
60 mM 0.0303 mL 0.1514 mL 0.3027 mL 0.7569 mL
80 mM 0.0227 mL 0.1135 mL 0.2271 mL 0.5677 mL
100 mM 0.0182 mL 0.0908 mL 0.1816 mL 0.4541 mL
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Liquiritin apioside
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