LPPM-8 

LPPM-8 is a ligand of Med25 and an inhibitor of Med25 protein-protein interactions (PPIs). LPPM-8 engages Med25 through interaction with the H2 face of its Activator Interaction Domain and stabilizes full-length protein in the cellular proteome. LPPM-8 is an orthosteric inhibitor of H2-binding transcriptional activators (such as ATF6a). LPPM-8 can be used for studying Med25 and Mediator complex biology^[1].

Med25 is a substoichiometric component of the megadalton Mediator complex and serves to bridge Mediator and transcriptional activators during transcription initiation through PPIs with the amphipathic ETV/PEA3 family (cell migration, motility), ATF6a (stress response), and VP16 (viral infection)^[1].
LPPM-8 (10 and 20 μM) inhibits a Med25-dependent gene (MMP-9) in VARIO68 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1012.28

C₅₀H₈₉N₇O₁₄

{(2S,3R)-3-Hydroxy-2-methylundecanoyl}-{d-(Glu-Asp-Leu-Leu-Leu-Leu-Val)}

{(2S,3R)-3-Hydroxy-2-methylundecanoyl}-{d-(EDLLLLV)}

CCCCCCCC[C@H]([C@@H](C(N[C@@H](C(N[C@@H](C(N[C@@H](C(N[C@@H](C(N[C@@H](C(N[C@@H](C(N[C@H](C(C)C)C(O)=O)=O)CC(C)C)=O)CC(C)C)=O)CC(C)C)=O)CC(C)C)=O)CC(O)=O)=O)CCC(O)=O)=O)C)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Pattelli ON, et al. A Lipopeptidomimetic of Transcriptional Activation Domains Selectively Disrupts Med25 Protein-Protein Interactions. Angew Chem Int Ed Engl. 2024 Mar 25:e202400781.  [Content Brief]