2. Apoptosis
  3. Apoptosis
  4. M50054

M50054 is a potent inhibitor of apoptosis. M50054 inhibits Etoposide-induced caspase-3 activation of U937 cells with an IC50 of 79 μg/mL. M50054 does not directly inhibit the enzymatic activity of caspase-3. M50054 can be used for the research anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia.

For research use only. We do not sell to patients.

M50054 Chemical Structure

M50054 Chemical Structure

CAS No. : 54135-60-3

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5 mg USD 117 In-stock
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M50054 Related Antibodies

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Description

M50054 is a potent inhibitor of apoptosis. M50054 inhibits Etoposide-induced caspase-3 activation of U937 cells with an IC50 of 79 μg/mL. M50054 does not directly inhibit the enzymatic activity of caspase-3. M50054 can be used for the research anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia[1].

In Vitro

M50054 inhibits the caspase-3 activation by Etoposide in a dose-dependent manner. The IC50 value of M50054 against Etoposide-induced caspase-3 activation of U937 cells is 79 μg/mL. However, M50054 does not directly inhibit the enzymatic activity of caspase-3 at all concentrations up to 1000 μg/mL[1].
M50054 (1-300 μg/mL) inhibits soluble human Fas ligand-induced cell death of human Fas-expressing WC8 cells in a dose-dependent manner, and the IC50 of 67 μg/mL[1].
M50054 (30-300 μg/mL) inhibits the death of U937 cells induced by etoposide (10 μg/mL) in a dose-dependent manner, and the IC50 value of M50054 against Etoposide-induced cell death of U937 cells is 130 μg/mL. The IC50 value of M50054 against etoposide-induced DNA fragmentation of U937 cells was 54 μg/mL[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

M50054 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Human Fas-expressing WC8 cells
Concentration: 1, 3, 10, 30, 100, 300 μg/mL
Incubation Time: Pre-incubated for 30 min
Result: Inhibited soluble human Fas ligand-induced cell death of human Fas-expressing WC8 cells in a dose-dependent manner, and the IC50 of 67 μg/mL.
In Vivo

M50054 (10-300 mg/kg; oral administration 30 min before anti-Fas antibody injection) inhibits the elevation of alanine aminotransferase and aspartate aminotransferase in a dose-dependent manner[1].
M50054 (topical treatment; given daily beginning on day 5 after birth and ending on day 14) significantly improves alopecia (hair loss) symptoms[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c mice[1]
Dosage: 10, 30, 100 or 300 mg/kg
Administration: Orally administered in a volume of 10 mL/kg
Result: Inhibited the elevation of alanine aminotransferase and aspartate aminotransferase in a dose-dependent manner.
Significantly improved these parameters at doses of 100 mg/kg and 300 mg/kg.
Animal Model: Five-day-old SD rats[1]
Dosage:
Administration: Topically applied to the head and back of 5-day-old rats once daily for 10 days
Result: Alopecia scores significantly decreased throughout the entire observation period compared with the control group.
Molecular Weight

236.26

Formula

C13H16O4
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C(CC(C(CCC2)=O)C2=O)C(CCC1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (423.26 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2326 mL 21.1631 mL 42.3263 mL
5 mM 0.8465 mL 4.2326 mL 8.4653 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2326 mL 21.1631 mL 42.3263 mL 105.8156 mL
5 mM 0.8465 mL 4.2326 mL 8.4653 mL 21.1631 mL
10 mM 0.4233 mL 2.1163 mL 4.2326 mL 10.5816 mL
15 mM 0.2822 mL 1.4109 mL 2.8218 mL 7.0544 mL
20 mM 0.2116 mL 1.0582 mL 2.1163 mL 5.2908 mL
25 mM 0.1693 mL 0.8465 mL 1.6931 mL 4.2326 mL
30 mM 0.1411 mL 0.7054 mL 1.4109 mL 3.5272 mL
40 mM 0.1058 mL 0.5291 mL 1.0582 mL 2.6454 mL
50 mM 0.0847 mL 0.4233 mL 0.8465 mL 2.1163 mL
60 mM 0.0705 mL 0.3527 mL 0.7054 mL 1.7636 mL
80 mM 0.0529 mL 0.2645 mL 0.5291 mL 1.3227 mL
100 mM 0.0423 mL 0.2116 mL 0.4233 mL 1.0582 mL
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M50054 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M5005454135-60-3M 50054M-50054ApoptosisFasEtoposideCaspase-3HepatitisAlopeciaanti-FaschemotherapyInhibitorinhibitorinhibit

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