2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. M5N36

M5N36 is a potent and selective Cdc25C inhibitor with IC50 values of 0.15, 0.19, 0.06 µM for Cdc25A, Cdc25B, Cdc25C, respectively. M5N36 shows anti-proliferative activity and increases the expression of p-CDK1 and p-CDK2.

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M5N36 Chemical Structure

M5N36 Chemical Structure

CAS No. : 2832887-40-6

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5 mg USD 125 In-stock
10 mg USD 200 In-stock
25 mg USD 410 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of M5N36:

M5N36 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

M5N36 is a potent and selective Cdc25C inhibitor with IC50 values of 0.15, 0.19, 0.06 µM for Cdc25A, Cdc25B, Cdc25C, respectively. M5N36 shows anti-proliferative activity and increases the expression of p-CDK1 and p-CDK2[1].

IC50 & Target

IC50: 0.15 µM (Cdc25A); 0.19 µM (Cdc25B); 0.06 µM (Cdc25C)[1]
In Vitro

M5N36 (5, 10 µM; 24 h) increases the expression of p-CDK1 and p-CDK2 in MDA-MB-231 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

M5N36 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Eca-109, HepG2, MCF-TAM, MDA-MB-231, Raji, SGC-7901, SW-620, HUVEC cells
Concentration:
Incubation Time:
Result: Showed anti-proliferative activity with IC50s of 2.88, 3.57, 10.00, 2.00, 1.20, 6.03, 3.39, 2.40 µM for Eca-109, HepG2, MCF-TAM, MDA-MB-231, Raji, SGC-7901, SW-620, HUVEC cells, respectively.

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 5, 10 µM
Incubation Time: 24 h
Result: Increased the expression of Cdc25A, Cdc25B, increased the expression of p-CDK1 and p-CDK2.
Molecular Weight

409.83

Formula

C20H16ClN5O3
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C1C(Cl)=C(C(C2=NC=CC=C21)=O)NC(C3=CN(N=N3)C4=CC=C(C=C4)CO)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (61.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4400 mL 12.2002 mL 24.4004 mL
5 mM 0.4880 mL 2.4400 mL 4.8801 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.10 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4400 mL 12.2002 mL 24.4004 mL 61.0009 mL
5 mM 0.4880 mL 2.4400 mL 4.8801 mL 12.2002 mL
10 mM 0.2440 mL 1.2200 mL 2.4400 mL 6.1001 mL
15 mM 0.1627 mL 0.8133 mL 1.6267 mL 4.0667 mL
20 mM 0.1220 mL 0.6100 mL 1.2200 mL 3.0500 mL
25 mM 0.0976 mL 0.4880 mL 0.9760 mL 2.4400 mL
30 mM 0.0813 mL 0.4067 mL 0.8133 mL 2.0334 mL
40 mM 0.0610 mL 0.3050 mL 0.6100 mL 1.5250 mL
50 mM 0.0488 mL 0.2440 mL 0.4880 mL 1.2200 mL
60 mM 0.0407 mL 0.2033 mL 0.4067 mL 1.0167 mL
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M5N36 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M5N362832887-40-6Phosphataseanti-proliferativep-CDK1p-CDK2MDA-MB-231 cellsanti-cancerCdc25 phosphataseInhibitorinhibitorinhibit

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