Maleimide

Name: Maleimide
Brand: MedChemExpress
SKU: HY-W007324
Rating: 4.5 (1 reviews)

Cat. No.: HY-W007324 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Maleimide can be used for production of antibody-drug conjugate (ADC) which is used in cancer research. Maleimide also be leveraged for the preparation of fluorogenic probe, which is mainly used for the specific detection of thiol analytes.

For research use only. We do not sell to patients.

Maleimide Chemical Structure

CAS No. : 541-59-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 25	In-stock	Estimated Time of Arrival: December 31
5 g	USD 50	In-stock	Estimated Time of Arrival: December 31
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50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

97.07

Formula

C₄H₃NO₂

CAS No.

541-59-3

Appearance

Solid

Color

White to light yellow

SMILES

O=C(N1)C=CC1=O

Structure Classification

Initial Source

Microorganisms

Endogenous metabolite

Streptomyces showdoensis

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (1030.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (515.09 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	10.3018 mL	51.5092 mL	103.0184 mL
5 mM	2.0604 mL	10.3018 mL	20.6037 mL
10 mM	1.0302 mL	5.1509 mL	10.3018 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (25.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (25.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (25.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 60 mg/mL (618.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (273 KB) SDS (642 KB)

COA (248 KB) HNMR (114 KB) RP-HPLC (256 KB)

Handling Instructions (2659 KB)

References

[1]. Renault K, et, al. Covalent Modification of Biomolecules through Maleimide-Based Labeling Strategies. Bioconjug Chem. 2018 Aug 15;29(8):2497-2513. [Content Brief]

[2]. Zhang S, et, al. A Maleimide-functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells. Chem Asian J. 2019 Mar 15;14(6):904-909. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	10.3018 mL	51.5092 mL	103.0184 mL	257.5461 mL
	5 mM	2.0604 mL	10.3018 mL	20.6037 mL	51.5092 mL
	10 mM	1.0302 mL	5.1509 mL	10.3018 mL	25.7546 mL
	15 mM	0.6868 mL	3.4339 mL	6.8679 mL	17.1697 mL
	20 mM	0.5151 mL	2.5755 mL	5.1509 mL	12.8773 mL
	25 mM	0.4121 mL	2.0604 mL	4.1207 mL	10.3018 mL
	30 mM	0.3434 mL	1.7170 mL	3.4339 mL	8.5849 mL
	40 mM	0.2575 mL	1.2877 mL	2.5755 mL	6.4387 mL
	50 mM	0.2060 mL	1.0302 mL	2.0604 mL	5.1509 mL
	60 mM	0.1717 mL	0.8585 mL	1.7170 mL	4.2924 mL
	80 mM	0.1288 mL	0.6439 mL	1.2877 mL	3.2193 mL
	100 mM	0.1030 mL	0.5151 mL	1.0302 mL	2.5755 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Maleimide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Maleimide541-59-3ADC LinkerAntibody-drug conjugates linkerantibody-drugconjugateADCcancerfluorogenicprobeInhibitorinhibitorinhibit

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Maleimide

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Maleimide Related Antibodies

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Maleimide Related Classifications

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