2. Metabolic Enzyme/Protease
  3. MMP
  4. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2  (Synonyms: Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2)

Cat. No.: HY-P4931 Purity: 99.87%
COA Handling Instructions

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Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1. (Ex/Em=325 nm/400 nm)

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Chemical Structure

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Chemical Structure

CAS No. : 720710-69-0

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1 mg USD 90 In-stock
5 mg USD 260 In-stock
10 mg USD 410 In-stock
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Based on 1 publication(s) in Google Scholar

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Related Antibodies

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View All MMP Isoform Specific Products:

View All Isoforms
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

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  • Purity & Documentation

  • References

  • Customer Review

Description

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1[1]. (Ex/Em=325 nm/400 nm)

IC50 & Target[1]

MMP-1

27.5 μM (Km)

MMP-8

 

MMP-13

5.2 μM (Km)

MMP-14

7.9 μM (Km)

In Vitro

Specificity of Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH26 for collagenases (MMP-1, MMP-8, MMP-13) and MT1-MMP (MMP-14) The constant (kcat/Km) increases by a factor of 2-9, or 3 times than FS-1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Related Antibodies
Molecular Weight

1221.32

Formula

C55H80N16O16
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Sequence

{Mca}-Lys-Pro-Leu-Gly-Leu-{Dap(Dnp)}-Ala-Arg-NH2
Sequence Shortening

{Mca}-KPLGL-{Dap(Dnp)}-AR-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (81.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (8.19 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8188 mL 4.0939 mL 8.1879 mL
5 mM 0.1638 mL 0.8188 mL 1.6376 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.87%

COA (253 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.8188 mL 4.0939 mL 8.1879 mL 20.4697 mL
5 mM 0.1638 mL 0.8188 mL 1.6376 mL 4.0939 mL
DMSO 10 mM 0.0819 mL 0.4094 mL 0.8188 mL 2.0470 mL
15 mM 0.0546 mL 0.2729 mL 0.5459 mL 1.3646 mL
20 mM 0.0409 mL 0.2047 mL 0.4094 mL 1.0235 mL
25 mM 0.0328 mL 0.1638 mL 0.3275 mL 0.8188 mL
30 mM 0.0273 mL 0.1365 mL 0.2729 mL 0.6823 mL
40 mM 0.0205 mL 0.1023 mL 0.2047 mL 0.5117 mL
50 mM 0.0164 mL 0.0819 mL 0.1638 mL 0.4094 mL
60 mM 0.0136 mL 0.0682 mL 0.1365 mL 0.3412 mL
80 mM 0.0102 mL 0.0512 mL 0.1023 mL 0.2559 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2720710-69-0Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2MMPMatrix metalloproteinasesfluorescent peptidequenched MMP peptide substratereal-time quantificationenzymatic activitycollagenasesMMP-1MMP-8MMP-13MT1-MMPInhibitorinhibitorinhibit

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