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MES sodium salt  (Synonyms: 2-Morpholinoethanesulphonic acid sodium salt)

Cat. No.: HY-D0858B Purity: ≥98.0%
Data Sheet SDS COA Handling Instructions

MES (2-Morpholinoethanesulphonic acid) sodium salt is a buffering agent in biology and biochemistry. MES sodium salt is one of the Good's buffers, the buffer capacity ranging pH 5.5-7.0. MES sodium salt is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments.

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MES sodium salt Chemical Structure

MES sodium salt Chemical Structure

CAS No. : 71119-23-8

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of MES sodium salt:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MES sodium salt

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MES (2-Morpholinoethanesulphonic acid) sodium salt is a buffering agent in biology and biochemistry. MES sodium salt is one of the Good's buffers, the buffer capacity ranging pH 5.5-7.0. MES sodium salt is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments[1][2].

Molecular Weight

217.22

Formula

C6H12NNaO4S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCOCC1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (115.09 mM; Need ultrasonic)

DMSO : 25 mg/mL (115.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6036 mL 23.0181 mL 46.0363 mL
5 mM 0.9207 mL 4.6036 mL 9.2073 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.6036 mL 23.0181 mL 46.0363 mL 115.0907 mL
5 mM 0.9207 mL 4.6036 mL 9.2073 mL 23.0181 mL
10 mM 0.4604 mL 2.3018 mL 4.6036 mL 11.5091 mL
15 mM 0.3069 mL 1.5345 mL 3.0691 mL 7.6727 mL
20 mM 0.2302 mL 1.1509 mL 2.3018 mL 5.7545 mL
25 mM 0.1841 mL 0.9207 mL 1.8415 mL 4.6036 mL
30 mM 0.1535 mL 0.7673 mL 1.5345 mL 3.8364 mL
40 mM 0.1151 mL 0.5755 mL 1.1509 mL 2.8773 mL
50 mM 0.0921 mL 0.4604 mL 0.9207 mL 2.3018 mL
60 mM 0.0767 mL 0.3836 mL 0.7673 mL 1.9182 mL
80 mM 0.0575 mL 0.2877 mL 0.5755 mL 1.4386 mL
100 mM 0.0460 mL 0.2302 mL 0.4604 mL 1.1509 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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MES sodium salt
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