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MES monohydrate  (Synonyms: 2-Morpholinoethanesulphonic acid monohydrate)

Cat. No.: HY-D0858A Purity: 99.87%
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MES (2-Morpholinoethanesulphonic acid) monohydrate is a buffering agent in biology and biochemistry. MES monohydrate is one of the Good's buffers, the buffer capacity ranging pH 5.5-7.0. MES monohydrate is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments.

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MES monohydrate Chemical Structure

MES monohydrate Chemical Structure

CAS No. : 145224-94-8

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Based on 1 publication(s) in Google Scholar

Other Forms of MES monohydrate:

MES monohydrate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MES monohydrate

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  • Purity & Documentation

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Description

MES (2-Morpholinoethanesulphonic acid) monohydrate is a buffering agent in biology and biochemistry. MES monohydrate is one of the Good's buffers, the buffer capacity ranging pH 5.5-7.0. MES monohydrate is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments[1][2].

In Vitro

Using MES Monohydrate to Regulate Arabidopsis Root Growth Experiment[2]
1. Solution Preparation
MES buffer solution:
(1) Prepare a 0.01%, 0.1%, or 1% MES monohydrate solution by adding 0.01 g, 0.1 g, or 1 g of MES monohydrate to 1 L of 1/2 MS medium, respectively.
(2) Adjust the pH of the solution to 5.8 using KOH.
(3) Sterilize the solution by autoclaving before use.
MES staining solution:
(1) When staining is required, prepare a 300 µM NBT solution for superoxide detection.
2. Experimental Procedure
(1) Seed sterilization: Sterilize Arabidopsis seeds using 2% NaClO and 0.1% Triton-X for 5 minutes, then rinse four times with Milli-Q water.
(2) Sowing: Sow the sterilized seeds on 1/2 MS medium containing different concentrations of MES (0.01%, 0.1%, 1%).
(3) Cold treatment: Place the plates at 4°C for 1 day after sowing.
(4) Cultivation: Place the plates vertically in a growth chamber at 23-25°C with a 16-hour light / 8-hour dark cycle.
3. Root Observation and Data Collection
(1) Root length measurement: On days 3 and 6, take images of the root tips using a microscope and measure the root length using ImageJ software.
(2) Root hair measurement: After 6 days, observe root hair formation under a microscope and measure the length of five root hairs, recording the average length.
(3) Superoxide detection: Stain the roots with 300 µM NBT solution for 5 minutes to detect superoxide production, and observe the stained areas under a light microscope.
4. Precautions
(1) pH control: Ensure the pH of the medium is maintained at 5.8 for optimal root growth conditions.
(2) Shaking conditions: During staining, gently shake the samples on a shaker to ensure uniform staining.
(3) Solution storage: Store the MES monohydrate solution after sterilization, preferably in a cold and dark environment for long-term use.
(4) Reuse: MES monohydrate buffer solution can be reused multiple times, but the pH and component stability should be checked before each experiment.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MES monohydrate Related Antibodies
Molecular Weight

213.25

Formula

C6H15NO5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCOCC1)(O)=O.[H]O[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool
Solvent & Solubility
In Vitro: 

DMSO : 8.75 mg/mL (41.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6893 mL 23.4467 mL 46.8933 mL
5 mM 0.9379 mL 4.6893 mL 9.3787 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.43 mg/mL (6.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.43 mg/mL (6.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.87%

COA (250 KB) HNMR (132 KB) RP-HPLC (208 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.6893 mL 23.4467 mL 46.8933 mL 117.2333 mL
5 mM 0.9379 mL 4.6893 mL 9.3787 mL 23.4467 mL
10 mM 0.4689 mL 2.3447 mL 4.6893 mL 11.7233 mL
15 mM 0.3126 mL 1.5631 mL 3.1262 mL 7.8156 mL
20 mM 0.2345 mL 1.1723 mL 2.3447 mL 5.8617 mL
25 mM 0.1876 mL 0.9379 mL 1.8757 mL 4.6893 mL
30 mM 0.1563 mL 0.7816 mL 1.5631 mL 3.9078 mL
40 mM 0.1172 mL 0.5862 mL 1.1723 mL 2.9308 mL
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MES monohydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MES145224-94-82-Morpholinoethanesulphonic acidBiochemical Assay ReagentsGood's buffersplants culture mediumreagent solutionphysiological experimentsInhibitorinhibitorinhibit

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