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MES  (Synonyms: 2-Morpholinoethanesulphonic acid)

Cat. No.: HY-D0858 Purity: 99.85%
Data Sheet
SDS
COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

MES (2-Morpholinoethanesulphonic acid) is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies.

For research use only. We do not sell to patients.

MES Chemical Structure

MES Chemical Structure

CAS No. : 4432-31-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
500 mg USD 25 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of MES:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MES

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MES (2-Morpholinoethanesulphonic acid) is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies[1][2].

In Vitro

MES is a zwitterionic buffer and can provide effective buffering capacity at around pH 6.15. The pKa value of MES changes with temperature, so the change of its pKa value needs to be considered when using it at different temperatures[1].
MES shows good buffering properties in biological experiments, exhibits poor affinity with metal ions (binding constants at 20 ℃ and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), thereby can be used in studies involving metal ions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

195.24

Formula

C6H13NO4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCOCC1)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (640.24 mM; Need ultrasonic)

DMSO : 50 mg/mL (256.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.1219 mL 25.6095 mL 51.2190 mL
5 mM 1.0244 mL 5.1219 mL 10.2438 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (256.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Purity: 99.93%

Data Sheet (272 KB) SDS (393 KB)

COA (247 KB) HNMR (261 KB) RP-HPLC (202 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 5.1219 mL 25.6095 mL 51.2190 mL 128.0475 mL
5 mM 1.0244 mL 5.1219 mL 10.2438 mL 25.6095 mL
10 mM 0.5122 mL 2.5610 mL 5.1219 mL 12.8048 mL
15 mM 0.3415 mL 1.7073 mL 3.4146 mL 8.5365 mL
20 mM 0.2561 mL 1.2805 mL 2.5610 mL 6.4024 mL
25 mM 0.2049 mL 1.0244 mL 2.0488 mL 5.1219 mL
30 mM 0.1707 mL 0.8537 mL 1.7073 mL 4.2683 mL
40 mM 0.1280 mL 0.6402 mL 1.2805 mL 3.2012 mL
50 mM 0.1024 mL 0.5122 mL 1.0244 mL 2.5610 mL
60 mM 0.0854 mL 0.4268 mL 0.8537 mL 2.1341 mL
80 mM 0.0640 mL 0.3201 mL 0.6402 mL 1.6006 mL
100 mM 0.0512 mL 0.2561 mL 0.5122 mL 1.2805 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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MES Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

MES4432-31-92-Morpholinoethanesulphonic acidBiochemical Assay ReagentsGood's buffersplants culture mediumreagent solutionphysiological experimentsInhibitorinhibitorinhibit

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