Methyl jasmonate

Cat. No.: HY-135663: FAQs
Data Sheet Handling Instructions

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Methyl jasmonate is a phytohormone involved in plant defenses under stress conditions. Methyl jasmonate can improve antioxidant properties of blueberry leaf extracts (mainly anthocyanins), and decrease the viability and migration capacity of AGS cells. Anticarcinogenic activity.

For research use only. We do not sell to patients.

Methyl jasmonate Chemical Structure

CAS No. : 1211-29-6

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Methyl jasmonate-treated blueberry leaf extraction (10-3200 μg/mL; 1 hour) can decrease cell viability of AGS cells with increasing dose^[1].
Methyl jasmonate-treated blueberry leaf extraction (10-3200 μg/mL) can significantly decrease the expression of p-P70S6K (AKT/mTOR pathway) and p-ERK1/2 (MAPK) proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Gastric cancer cells (line AGS)^[1]
Concentration:	10, 25, 50, 100, 200, 400, 800, 1600 and 3200 μg/mL
Incubation Time:	1 hour
Result:	Cell viability of AGS cells gradually decreased in response to increasing doses of blueberry leaf extract from MeJA treated plants.

Western Blot Analysis

Cell Line:	AGS cells^[1]
Concentration:	10, 50, 100, 200, 400, 800, 1600 and 3200 μg/mL
Incubation Time:
Result:	Blueberry leaf extracts of MeJA-treated plants significantly decreased the expression of p-P70S6K (AKT/mTOR pathway) and p-ERK1/2 (MAPK) proteins.

Molecular Weight

224.30

Formula

C₁₃H₂₀O₃

CAS No.

1211-29-6

Appearance

Liquid

SMILES

O=C(OC)C[C@@H]1[C@@H](C/C=C\CC)C(CC1)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (445.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4583 mL	22.2916 mL	44.5831 mL
5 mM	0.8917 mL	4.4583 mL	8.9166 mL
10 mM	0.4458 mL	2.2292 mL	4.4583 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Ribera-Fonseca A, et al. The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties. Antioxidants (Basel). 2020;9(1):45. Published 2020 Jan 4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4583 mL	22.2916 mL	44.5831 mL	111.4579 mL
	5 mM	0.8917 mL	4.4583 mL	8.9166 mL	22.2916 mL
	10 mM	0.4458 mL	2.2292 mL	4.4583 mL	11.1458 mL
	15 mM	0.2972 mL	1.4861 mL	2.9722 mL	7.4305 mL
	20 mM	0.2229 mL	1.1146 mL	2.2292 mL	5.5729 mL
	25 mM	0.1783 mL	0.8917 mL	1.7833 mL	4.4583 mL
	30 mM	0.1486 mL	0.7431 mL	1.4861 mL	3.7153 mL
	40 mM	0.1115 mL	0.5573 mL	1.1146 mL	2.7864 mL
	50 mM	0.0892 mL	0.4458 mL	0.8917 mL	2.2292 mL
	60 mM	0.0743 mL	0.3715 mL	0.7431 mL	1.8576 mL
	80 mM	0.0557 mL	0.2786 mL	0.5573 mL	1.3932 mL
	100 mM	0.0446 mL	0.2229 mL	0.4458 mL	1.1146 mL