MI-2-2

Name: MI-2-2
Brand: MedChemExpress
SKU: HY-108350
Rating: 4.5 (1 reviews)

Cat. No.: HY-108350 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MI-2-2 is a potent menin-MLL inhibitor. MI-2-2 binds to menin with low nanomolar affinity (K_d=22nM) and very effectively disrupts the bivalent protein-protein interaction between menin and MLL. MI-2-2 has specific and very pronounced activity in MLL leukemia cells, including inhibition of cell proliferation, down-regulation of Hoxa9 expression, and differentiation.

For research use only. We do not sell to patients.

MI-2-2 Chemical Structure

CAS No. : 1454920-20-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 319	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 319	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
K562	IC₅₀	> 20 μM Compound: 1; MI-2-2	Antiproliferative activity against human K562 cells after 7 days by MTS assay Antiproliferative activity against human K562 cells after 7 days by MTS assay	[PMID: 27528435]
MV4-11	IC₅₀	0.64 μM Compound: 1; MI-2-2	Antiproliferative activity against human MV4-11 cells expressing MLL-AF4 after 7 days by MTS assay Antiproliferative activity against human MV4-11 cells expressing MLL-AF4 after 7 days by MTS assay	[PMID: 27528435]
MV4-11	IC₅₀	2.6 μM Compound: MI-2-2	Antiproliferative activity against human MV4-11 cells after 168 hrs by MTS assay Antiproliferative activity against human MV4-11 cells after 168 hrs by MTS assay	[PMID: 30713023]

In Vitro

MI-2-2 is capable of inhibiting both the interaction of menin with MBM1 (IC₅₀= 46 nM) and with the bivalent fragment of MLL that comprises both MBM1 and MBM2 (IC₅₀=520 nM). MI-2-2 exhibits very pronounced activities at low micromolar concentrations in BMCs transformed with MLL-AF9 and in MV4;11, a human leukemia cell line harboring the MLL-AF4 translocation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

415.50

Formula

C₁₇H₂₀F₃N₅S₂

CAS No.

1454920-20-7

Appearance

Solid

Color

White to off-white

SMILES

FC(F)(F)CC1=CC2=C(N3CCN(C4=NCC(C)(C)S4)CC3)N=CN=C2S1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (240.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4067 mL	12.0337 mL	24.0674 mL
5 mM	0.4813 mL	2.4067 mL	4.8135 mL
10 mM	0.2407 mL	1.2034 mL	2.4067 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.75 mg/mL (9.03 mM); Clear solution

This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (249 KB) HNMR (287 KB) LCMS (130 KB)

Handling Instructions (2659 KB)

References

[1]. Shi A, et al. Structural insights into inhibition of the bivalent menin-MLL interaction by small molecules in leukemia. Blood. 2012;120(23):4461-4469. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4067 mL	12.0337 mL	24.0674 mL	60.1685 mL
	5 mM	0.4813 mL	2.4067 mL	4.8135 mL	12.0337 mL
	10 mM	0.2407 mL	1.2034 mL	2.4067 mL	6.0168 mL
	15 mM	0.1604 mL	0.8022 mL	1.6045 mL	4.0112 mL
	20 mM	0.1203 mL	0.6017 mL	1.2034 mL	3.0084 mL
	25 mM	0.0963 mL	0.4813 mL	0.9627 mL	2.4067 mL
	30 mM	0.0802 mL	0.4011 mL	0.8022 mL	2.0056 mL
	40 mM	0.0602 mL	0.3008 mL	0.6017 mL	1.5042 mL
	50 mM	0.0481 mL	0.2407 mL	0.4813 mL	1.2034 mL
	60 mM	0.0401 mL	0.2006 mL	0.4011 mL	1.0028 mL
	80 mM	0.0301 mL	0.1504 mL	0.3008 mL	0.7521 mL
	100 mM	0.0241 mL	0.1203 mL	0.2407 mL	0.6017 mL

MI-2-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MI-2-21454920-20-7Epigenetic Reader Domainleukemiamenin-MLL interactioncell proliferationHoxa9 expressiondifferentiationInhibitorinhibitorinhibit

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MI-2-2

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MI-2-2 Related Antibodies

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

MI-2-2 Related Classifications

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