Miconazole (Synonyms: R18134)

Name: Miconazole
Brand: MedChemExpress
SKU: HY-B0454
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0454 Purity: 99.85%: FAQs
Data Sheet SDS COA Handling Instructions

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Miconazole (R18134) is an imidazole antifungal agent. Miconazole also has antibacterial effects.

For research use only. We do not sell to patients.

Miconazole Chemical Structure

CAS No. : 22916-47-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

Other Forms of Miconazole:

3 Publications Citing Use of MCE Miconazole

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Miconazole (R18134) is an imidazole antifungal agent. Miconazole also has antibacterial effects^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	6.5 μM Compound: Miconazole	Antagonist activity at human recombinant GluR6 expressed in HEK cells coexpressing aequorine assessed as inhibition of glutamate-induced Ca2+ influx at 10 uM by luminescence reporter assay Antagonist activity at human recombinant GluR6 expressed in HEK cells coexpressing aequorine assessed as inhibition of glutamate-induced Ca2+ influx at 10 uM by luminescence reporter assay	[PMID: 19819046]
HEK-293T	IC₅₀	17.8 μM Compound: Miconazole	Inhibition of mouse Ido1 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis Inhibition of mouse Ido1 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis	[PMID: 23122865]
HEK-293T	IC₅₀	6.7 μM Compound: Miconazole	Inhibition of mouse Ido2 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis Inhibition of mouse Ido2 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis	[PMID: 23122865]
Hepatocyte	IC₅₀	2.03 nM Compound: Miconazole	Antimicrobial activity against Plasmodium yoelii 265 liver infected in mammalian hepatocytes after 48 hrs Antimicrobial activity against Plasmodium yoelii 265 liver infected in mammalian hepatocytes after 48 hrs	[PMID: 18212104]
HL-60	IC₅₀	0.8 μM Compound: Miconazole	Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 33713977]
J774.A1	CC₅₀	9.93 μM Compound: Miconazole	Cytotoxicity against mouse J774A1 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against mouse J774A1 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 21385661]
K562	CC₅₀	80 μM Compound: 4, miconazole	Cytotoxicity against human K562 cells after assessed as reduction of cell viability 24 hrs by MTT assay Cytotoxicity against human K562 cells after assessed as reduction of cell viability 24 hrs by MTT assay	[PMID: 18529046]
LLC-PK1	IC₅₀	2 μM Compound: Miconazole	Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	3.5 μM Compound: Miconazole	Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	7.8 μM Compound: Miconazole	Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
MDA-MB-231	IC₅₀	100 μM Compound: 6	Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction of SERCA inhibition-induced ER release preincubated for 15 mins followed by CPA addition by PBX-based FLIPR assay Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction of SERCA inhibition-induced ER release preincubated for 15 mins followed by CPA addition by PBX-based FLIPR assay	[PMID: 27856238]
MDA-MB-231	IC₅₀	16.96 μM Compound: Miconazole	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 33713977]
MDA-MB-231	IC₅₀	27 μM Compound: 6	Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction in BAPTA-induced Ca2+ depletion-stimulated SOCE activity preincubated for 15 mins followed by BAPTA addition in presence of extracellular Ca2+ by PBX-ba Inhibition of Orai1-mediated store operated Ca2+ entry in human MDA-MB-231 cells assessed as reduction in BAPTA-induced Ca2+ depletion-stimulated SOCE activity preincubated for 15 mins followed by BAPTA addition in presence of extracellular Ca2+ by PBX-ba	[PMID: 27856238]
MRC5	IC₅₀	61.7 μg/mL Compound: MCZ	Cytotoxicity against human MRC5 cells incubated for 48 hrs by MTT assay Cytotoxicity against human MRC5 cells incubated for 48 hrs by MTT assay	[PMID: 31191871]
NHDF	GI₅₀	10.3 μM Compound: Miconazole	Cytotoxicity against NHDF assessed as reduction in cell viability after 5 days by CellTiter-Glo assay Cytotoxicity against NHDF assessed as reduction in cell viability after 5 days by CellTiter-Glo assay	[PMID: 29549835]
PC-3	IC₅₀	13.41 μM Compound: Miconazole	Cytotoxicity against human PC3 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 33713977]
U-937	CC₅₀	120 μM Compound: 4, miconazole	Cytotoxicity against human U937 cells assessed as reduction of cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as reduction of cell viability after 24 hrs by MTT assay	[PMID: 18529046]
Vero	CC₅₀	37.4 μM Compound: Miconazole	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23582274]

In Vitro

Miconazole is an imidazole antifungal agent, developed by Janssen Pharmaceutica, commonly applied topically to the skin or to mucous membranes to cure fungal infections. It works by inhibiting the synthesis of ergosterol, a critical component of fungal cell membranes. It can also be used against certain species of Leishmania protozoa which are a type of unicellular parasite that also contain ergosterol in their cell membranes. In addition to its antifungal and antiparasitic actions, it also has some antibacterial properties. Miconazole is also used in Ektachrome film developing in the final rinse of the Kodak E-6 process and similar Fuji CR-56 process, replacing formaldehyde. Fuji Hunt also includes miconazole as a final rinse additive in their formulation of the C-41RA rapid access color negative developing process. From Wikipedia.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

416.13

Formula

C₁₈H₁₄Cl₄N₂O

CAS No.

22916-47-8

Appearance

Solid

Color

White to off-white

SMILES

ClC1=C(C=CC(Cl)=C1)C(OCC(C=CC(Cl)=C2)=C2Cl)CN3C=CN=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (240.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4031 mL	12.0155 mL	24.0310 mL
5 mM	0.4806 mL	2.4031 mL	4.8062 mL
10 mM	0.2403 mL	1.2015 mL	2.4031 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.00 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (252 KB) HNMR (266 KB) RP-HPLC (246 KB) LCMS (261 KB)

Handling Instructions (2659 KB)

References

[1]. http://en.wikipedia.org/wiki/Miconazole

[2]. Nenoff P, et al. New insights on the antibacterial efficacy of miconazole in vitro. Mycoses. 2017 Aug;60(8):552-557. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4031 mL	12.0155 mL	24.0310 mL	60.0774 mL
	5 mM	0.4806 mL	2.4031 mL	4.8062 mL	12.0155 mL
	10 mM	0.2403 mL	1.2015 mL	2.4031 mL	6.0077 mL
	15 mM	0.1602 mL	0.8010 mL	1.6021 mL	4.0052 mL
	20 mM	0.1202 mL	0.6008 mL	1.2015 mL	3.0039 mL
	25 mM	0.0961 mL	0.4806 mL	0.9612 mL	2.4031 mL
	30 mM	0.0801 mL	0.4005 mL	0.8010 mL	2.0026 mL
	40 mM	0.0601 mL	0.3004 mL	0.6008 mL	1.5019 mL
	50 mM	0.0481 mL	0.2403 mL	0.4806 mL	1.2015 mL
	60 mM	0.0401 mL	0.2003 mL	0.4005 mL	1.0013 mL
	80 mM	0.0300 mL	0.1502 mL	0.3004 mL	0.7510 mL
	100 mM	0.0240 mL	0.1202 mL	0.2403 mL	0.6008 mL

Miconazole Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Miconazole22916-47-8R18134R 18134R-18134FungalBacterialAntibioticInhibitorinhibitorinhibit

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Miconazole (Synonyms: R18134)

Customer Review

Other Forms of Miconazole:

Miconazole Related Antibodies

3 Publications Citing Use of MCE Miconazole

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Miconazole Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Miconazole Related Classifications

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