2. Others Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Fluorescent Dye Reactive Oxygen Species Mitochondrial Metabolism
  4. MitoSOX Red

MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red.
Excitation/emission wavelength: 510/580 nm.

For research use only. We do not sell to patients.

CAS No. : 1003197-00-9

Size Price Stock Quantity
50 μg In-stock
100 μg In-stock
500 μg In-stock

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 114 publication(s) in Google Scholar

Top Publications Citing Use of Products

107 Publications Citing Use of MCE MitoSOX Red

Mitochondrial ROS Imaging

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Oct 15.

    MitoSOX Red (5 µM). Mitochondrial ROS in H2O2-stimulated fibroblasts incubated with different hydrogels, assessed by DCFH-DA and MitoSOX staining, respectively.

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: Small. 2024 May 9:e2309891.  [Abstract]

    CLSM images of changes in ROS in mitochondria of HOS cells treated with different groups.

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Mar 30, 103143.

    MitoSOX Red (5 μM; 30 min). Results of incubation with the JC-1 fluorescent probe, FerroOrange probe, DCFH-DA, MitoSOX Red, and corresponding flow cytometric assays (#aP = 0.0014) are shown.

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Mar 25;22(1):129.  [Abstract]

    MitoSOX Red (10 μM; 30 min). Mitochondrial superoxide levels by MitoSOX Red fluorescent staining with MFI quantification. (G&H) Mitochondrial membrane potential by JC-1 staining with calculation of the ratio of red MFI (aggregated JC-1) to green MFI (monomer JC-1).

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2024 Aug 18:e2401909.  [Abstract]

    MitoSOX Red (5 μM; 30 min). Mitochondrial ROS detected by Mito-SOX RED in control and MCT-PASMCs with or without MPP nanoparticles. Scale bar: 100 µm.

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: Biomaterials. 2023 Aug 26, 122295.

    MitoSOX Red (30 min). Representative fluorescent images of MitoSox in 4T1and LLC cells.

    MitoSOX Red purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2023 Jun 13;457:131840.  [Abstract]

    MitoSOX Red (100 nM; 2 h). Representative images of Mito-Tracker Red-staining in P0C. elegans after nanoplastic exposure with different surface charges.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red.
    Excitation/emission wavelength: 510/580 nm.

    In Vitro

    General Protocol

    1. Preparation of MitoSOX Red working solution
    1.1 Preparation of the stock solution
    Dissolve 50 μg MitoSOX Red in 13 μL DMSO to obtain 5 mM of stock solution.
    Note: It is recommended to store the stock solution at -20°C and -80°C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of MitoSOX Red working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
    Note: Please adjust the concentration of MitoSOX Red working solution according to the actual situation.
    2. Cell staining
    2.1 Suspension cells（6-well plate）
    a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    d. Wash twice with PBS, 5 minutes each time.
    e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a. Culture adherent cells on sterile coverslips.
    b. Remove the coverslip from the medium and aspirate excess medium.
    c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
    d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

    Storage
    -20°C, 1 year. Protect from light

    Precautions
    1. Please adjust the concentration of MitoSOX Red working solution according to the actual situation.
    2. This product is for R&D use only, not for drug, household, or other uses.
    3. For your safety and health, please wear a lab coat and disposable gloves to operate.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    759.70

    Formula

    C43H43IN3P
    CAS No.
    Appearance

    Solid

    Color

    Yellow to brown

    Emission (Em)

    580
    Excitation (Ex)

    510
    SMILES

    NC1=CC(C(C2=CC=CC=C2)N(CCCCCC[P+](C3=CC=CC=C3)(C4=CC=CC=C4)C5=CC=CC=C5)C6=CC(N)=CC=C76)=C7C=C1.[I-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (131.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3163 mL 6.5815 mL 13.1631 mL
    5 mM 0.2633 mL 1.3163 mL 2.6326 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.29 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 95.58%

    Data Sheet (280 KB) SDS (252 KB)

    COA (250 KB) HNMR (280 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.3163 mL 6.5815 mL 13.1631 mL 32.9077 mL
    5 mM 0.2633 mL 1.3163 mL 2.6326 mL 6.5815 mL
    10 mM 0.1316 mL 0.6582 mL 1.3163 mL 3.2908 mL
    15 mM 0.0878 mL 0.4388 mL 0.8775 mL 2.1938 mL
    20 mM 0.0658 mL 0.3291 mL 0.6582 mL 1.6454 mL
    25 mM 0.0527 mL 0.2633 mL 0.5265 mL 1.3163 mL
    30 mM 0.0439 mL 0.2194 mL 0.4388 mL 1.0969 mL
    40 mM 0.0329 mL 0.1645 mL 0.3291 mL 0.8227 mL
    50 mM 0.0263 mL 0.1316 mL 0.2633 mL 0.6582 mL
    60 mM 0.0219 mL 0.1097 mL 0.2194 mL 0.5485 mL
    80 mM 0.0165 mL 0.0823 mL 0.1645 mL 0.4113 mL
    100 mM 0.0132 mL 0.0658 mL 0.1316 mL 0.3291 mL
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    MitoSOX Red Related Classifications

    Keywords:

    MitoSOX Red1003197-00-9Fluorescent DyeReactive Oxygen SpeciesMitochondrial MetabolismMitochondrial ROS Indicatorfluorescent dyemitochondrial ROSInhibitorinhibitorinhibit

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