MRGPRX4 modulator-1

Name: MRGPRX4 modulator-1
Brand: MedChemExpress
SKU: HY-145997
Rating: 4.5 (1 reviews)

Cat. No.: HY-145997 Purity: 99.09%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MRGPRX4 modulator-1 (compound 31-2) is a potent mas-related g-protein receptor X4 (MRGPR X4) modulator, with antagonist activity of IC₅₀ < 100 nM for MRGPR4. MRGPRX4 modulator-1 can be used for researching MRGPR X4 dependent diseases such as itch, pain, and autoimmune disorders.

For research use only. We do not sell to patients.

MRGPRX4 modulator-1 Chemical Structure

CAS No. : 2492596-61-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 222	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 222	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC₅₀: < 100 nM (MRGPR X4)^[1]

Molecular Weight

359.73

Formula

C₁₆H₁₃ClF₃NO₃

CAS No.

2492596-61-7

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=NC(COC2=CC=C(C(F)(F)F)C=C2Cl)=CC(CC)=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (277.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7799 mL	13.8993 mL	27.7986 mL
5 mM	0.5560 mL	2.7799 mL	5.5597 mL
10 mM	0.2780 mL	1.3899 mL	2.7799 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.09%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (246 KB) HNMR (117 KB) RP-HPLC (169 KB) LCMS (358 KB)

Handling Instructions (2659 KB)

References

[1]. Adam YEAGER, et al. Modulators of mas-related g-protein receptor x4 and related products and methods. WO2020198537 (compound 31-2).

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7799 mL	13.8993 mL	27.7986 mL	69.4966 mL
	5 mM	0.5560 mL	2.7799 mL	5.5597 mL	13.8993 mL
	10 mM	0.2780 mL	1.3899 mL	2.7799 mL	6.9497 mL
	15 mM	0.1853 mL	0.9266 mL	1.8532 mL	4.6331 mL
	20 mM	0.1390 mL	0.6950 mL	1.3899 mL	3.4748 mL
	25 mM	0.1112 mL	0.5560 mL	1.1119 mL	2.7799 mL
	30 mM	0.0927 mL	0.4633 mL	0.9266 mL	2.3166 mL
	40 mM	0.0695 mL	0.3475 mL	0.6950 mL	1.7374 mL
	50 mM	0.0556 mL	0.2780 mL	0.5560 mL	1.3899 mL
	60 mM	0.0463 mL	0.2317 mL	0.4633 mL	1.1583 mL
	80 mM	0.0347 mL	0.1737 mL	0.3475 mL	0.8687 mL
	100 mM	0.0278 mL	0.1390 mL	0.2780 mL	0.6950 mL