MRT-83 hydrochloride

Name: MRT-83 hydrochloride
Brand: MedChemExpress
SKU: HY-18287A
Rating: 4.5 (1 reviews)

Cat. No.: HY-18287A Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MRT-83 (hydrochloride) is the potent antagonist of Smoothened (Smo) receptor. MRT-83 (hydrochloride) inhibits the Hedgehog (Hh) signaling pathway and BODIPY-cyclopamine binding to human Smo. MRT-83 (hydrochloride) has the potential for researching cancer disease.

For research use only. We do not sell to patients.

MRT-83 hydrochloride Chemical Structure

CAS No. : 1359944-60-7

Size	Price	Stock	Quantity
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 390	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 670	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of MRT-83 hydrochloride:

MRT-83 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Smo^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HT-1080	IC₅₀	29 μM Compound: 4; ST8117	Antiproliferative activity against human HT1080 cells after 72 hrs by sulforhodamine-B assay Antiproliferative activity against human HT1080 cells after 72 hrs by sulforhodamine-B assay	[PMID: 30099257]
LS180	IC₅₀	9.7 μM Compound: 4; ST8117	Antiproliferative activity against human LS180 cells after 72 hrs by sulforhodamine-B assay Antiproliferative activity against human LS180 cells after 72 hrs by sulforhodamine-B assay	[PMID: 30099257]

In Vitro

MRT-83 (hydrochloride) (compound 86) has IC₅₀s of 15 and 11 nM respectively in Shh-light2 and C3H10T1/2 cells in the biological activity assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

575.05

Formula

C₃₁H₃₁ClN₄O₅

CAS No.

1359944-60-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(C2=CC=CC=C2)C=C1)NC3=CC(NC(NC(C4=CC(OC)=C(OC)C(OC)=C4)=O)=N)=CC=C3C.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (434.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7390 mL	8.6949 mL	17.3898 mL
5 mM	0.3478 mL	1.7390 mL	3.4780 mL
10 mM	0.1739 mL	0.8695 mL	1.7390 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (246 KB) HNMR (574 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Solinas A, et al. Acylthiourea, acylurea, and acylguanidine derivatives with potent hedgehog inhibiting activity. J Med Chem. 2012;55(4):1559-1571. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7390 mL	8.6949 mL	17.3898 mL	43.4745 mL
	5 mM	0.3478 mL	1.7390 mL	3.4780 mL	8.6949 mL
	10 mM	0.1739 mL	0.8695 mL	1.7390 mL	4.3474 mL
	15 mM	0.1159 mL	0.5797 mL	1.1593 mL	2.8983 mL
	20 mM	0.0869 mL	0.4347 mL	0.8695 mL	2.1737 mL
	25 mM	0.0696 mL	0.3478 mL	0.6956 mL	1.7390 mL
	30 mM	0.0580 mL	0.2898 mL	0.5797 mL	1.4491 mL
	40 mM	0.0435 mL	0.2174 mL	0.4347 mL	1.0869 mL
	50 mM	0.0348 mL	0.1739 mL	0.3478 mL	0.8695 mL
	60 mM	0.0290 mL	0.1449 mL	0.2898 mL	0.7246 mL
	80 mM	0.0217 mL	0.1087 mL	0.2174 mL	0.5434 mL
	100 mM	0.0174 mL	0.0869 mL	0.1739 mL	0.4347 mL