MV1

Name: MV1
Brand: MedChemExpress
SKU: HY-113534
Rating: 4.5 (1 reviews)

Cat. No.: HY-113534 Purity: 98.47%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MV1 is an antagonist of IAP (inhibitor of apoptosis protein), leads to protein knockdown of HaloTag-fused proteins when combined with HaloTag ligand.

For research use only. We do not sell to patients.

MV1 Chemical Structure

CAS No. : 1001600-54-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 235	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 235	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 185	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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cIAP cIAP-1 cIAP-2 XIAP

Biological Activity
Purity & Documentation
References
Customer Review

Description

MV1 is an antagonist of IAP (inhibitor of apoptosis protein), leads to protein knockdown of HaloTag-fused proteins when combined with HaloTag ligand^[1].

In Vitro

MV1 (0.08-20 μM; 24 h) inhibits the growth of EVSAT cells^[1].
MV1 (5 μM; 0-60 min) treatment causes rapid loss of c-IAP1 and c-IAP2 in MDA-MB-231 cells^[1].
MV1 (5 μM; 1 h) treatment inducing degradation of c-IAP1 and c-IAP2 is dependent on proteasomal machinery but not on caspase activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	EVSAT cells
Concentration:	0.08-20 μM
Incubation Time:	24 hours
Result:	Showed IC₅₀ value of 5 μM for EVSAT cells.

Western Blot Analysis^[1]

Cell Line:	MDA-MB-231 cells
Concentration:	5 μM
Incubation Time:	0-60 min
Result:	Showed the decreasement of c-IAP1 and 2 protein levels by as early as two minutes following exposure.

Molecular Weight

576.73

Formula

C₃₃H₄₄N₄O₅

CAS No.

1001600-54-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(OC)[C@H](C(C1=CC=CC=C1)C2=CC=CC=C2)NC([C@H]3N(C([C@H](C4CCCCC4)NC([C@H](C)NC)=O)=O)CCC3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 125 mg/mL (216.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7339 mL	8.6696 mL	17.3391 mL
5 mM	0.3468 mL	1.7339 mL	3.4678 mL
10 mM	0.1734 mL	0.8670 mL	1.7339 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.47%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (255 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Tomoshige S, et al. Efficient protein knockdown of HaloTag-fused proteins using hybrid molecules consisting of IAP antagonist and HaloTag ligand. Bioorg Med Chem. 2016 Jul 15;24(14):3144-8. [Content Brief]

[2]. Eugene Varfolomeev, et al. IAP antagonists induce autoubiquitination of c-IAPs, NF-kappaB activation, and TNFalpha-dependent apoptosis. Cell. 2007 Nov 16;131(4):669-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7339 mL	8.6696 mL	17.3391 mL	43.3478 mL
	5 mM	0.3468 mL	1.7339 mL	3.4678 mL	8.6696 mL
	10 mM	0.1734 mL	0.8670 mL	1.7339 mL	4.3348 mL
	15 mM	0.1156 mL	0.5780 mL	1.1559 mL	2.8899 mL
	20 mM	0.0867 mL	0.4335 mL	0.8670 mL	2.1674 mL
	25 mM	0.0694 mL	0.3468 mL	0.6936 mL	1.7339 mL
	30 mM	0.0578 mL	0.2890 mL	0.5780 mL	1.4449 mL
	40 mM	0.0433 mL	0.2167 mL	0.4335 mL	1.0837 mL
	50 mM	0.0347 mL	0.1734 mL	0.3468 mL	0.8670 mL
	60 mM	0.0289 mL	0.1445 mL	0.2890 mL	0.7225 mL
	80 mM	0.0217 mL	0.1084 mL	0.2167 mL	0.5418 mL
	100 mM	0.0173 mL	0.0867 mL	0.1734 mL	0.4335 mL

MV1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MV11001600-54-9MV 1MV-1IAPApoptosisinhibitor of apoptosis proteinMDA-MB-231EVSATInhibitorinhibitorinhibit

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MV1

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MV1 Related Antibodies

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MV1 Related Antibodies

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Complete Stock Solution Preparation Table

MV1 Related Classifications

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