N-3-Oxo-octanoyl-L-homoserine lactone

Name: N-3-Oxo-octanoyl-L-homoserine lactone
Brand: MedChemExpress
SKU: HY-108700
Rating: 4.5 (1 reviews)

Cat. No.: HY-108700 Purity: 98.05%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N-3-Oxo-octanoyl-L-homoserine lactone, a quorum-sensing signal, is an Agrobacterium autoinducer.

For research use only. We do not sell to patients.

N-3-Oxo-octanoyl-L-homoserine lactone Chemical Structure

CAS No. : 147795-39-9

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

N-3-Oxo-octanoyl-L-homoserine lactone, a quorum-sensing signal, is an Agrobacterium autoinducer^[1]^[2].

In Vitro

N-3-Oxo-octanoyl-L-homoserine lactone (3OC8-HSL) pretreatment (10?μM; for 2?days) exerts strong inhibitory effect on pathogen proliferation in the leaves^[1].
Plants treated with N-3-Oxo-octanoyl-L-homoserine lactone shows increased activities of defense-related enzymes including peroxidase, catalase, phenylalanine ammonialyase, and superoxide dismutase^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

241.28

Formula

C₁₂H₁₉NO₄

CAS No.

147795-39-9

Appearance

Solid

Color

White to off-white

SMILES

CCCCCC(CC(N[C@@H]1C(OCC1)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (414.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1446 mL	20.7228 mL	41.4456 mL
5 mM	0.8289 mL	4.1446 mL	8.2891 mL
10 mM	0.4145 mL	2.0723 mL	4.1446 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.05%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (245 KB) HNMR (243 KB) RP-HPLC (193 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Fang Liu, et al. N-3-oxo-octanoyl-homoserine lactone-mediated priming of resistance to Pseudomonas syringae requires the salicylic acid signaling pathway in Arabidopsis thaliana. BMC Plant Biol. 2020 Jan 28;20(1):38. [Content Brief]

[2]. Hai-Bao Zhang, et al. Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1446 mL	20.7228 mL	41.4456 mL	103.6141 mL
	5 mM	0.8289 mL	4.1446 mL	8.2891 mL	20.7228 mL
	10 mM	0.4145 mL	2.0723 mL	4.1446 mL	10.3614 mL
	15 mM	0.2763 mL	1.3815 mL	2.7630 mL	6.9076 mL
	20 mM	0.2072 mL	1.0361 mL	2.0723 mL	5.1807 mL
	25 mM	0.1658 mL	0.8289 mL	1.6578 mL	4.1446 mL
	30 mM	0.1382 mL	0.6908 mL	1.3815 mL	3.4538 mL
	40 mM	0.1036 mL	0.5181 mL	1.0361 mL	2.5904 mL
	50 mM	0.0829 mL	0.4145 mL	0.8289 mL	2.0723 mL
	60 mM	0.0691 mL	0.3454 mL	0.6908 mL	1.7269 mL
	80 mM	0.0518 mL	0.2590 mL	0.5181 mL	1.2952 mL
	100 mM	0.0414 mL	0.2072 mL	0.4145 mL	1.0361 mL