N-Acetyl-L-histidine monohydrate

Name: N-Acetyl-L-histidine monohydrate
Brand: MedChemExpress
SKU: HY-W014180
Rating: 4.5 (1 reviews)

Cat. No.: HY-W014180 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N-Acetyl-L-histidine monohydrate, a histidine derivative, is a prominent biomolecule in brain, retina and lens of poikilothermic vertebrates. N-Acetyl-L-histidine monohydrate has a role as an animal metabolite.

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N-Acetyl-L-histidine monohydrate Chemical Structure

CAS No. : 39145-52-3

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Based on 1 publication(s) in Google Scholar

Other Forms of N-Acetyl-L-histidine monohydrate:

N-Acetylhistidine Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

N-Acetyl-L-histidine monohydrate (NAH) also exhibits a strong phylogenetic component in that it is a major osmolyte in the brain and eye of teleost (bony) fish, amphibians and reptiles, but is present in much lower amounts in brain and other tissues of homeothermic (endothermic) vertebrates^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

215.21

Formula

C₈H₁₃N₃O₄

CAS No.

39145-52-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@H](CC1=CNC=N1)NC(C)=O.[H]O[H]

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Animals

Endogenous metabolite

poikilothermic vertebrates

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 62.5 mg/mL (290.41 mM; ultrasonic and warming and heat to 60°C)

DMSO : 20 mg/mL (92.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6466 mL	23.2331 mL	46.4662 mL
5 mM	0.9293 mL	4.6466 mL	9.2932 mL
10 mM	0.4647 mL	2.3233 mL	4.6466 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (9.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (9.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (9.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 12.5 mg/mL (58.08 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (251 KB) HNMR (249 KB) RP-HPLC (263 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Morris H Baslow, et al. N-acetyl-L-histidine, a Prominent Biomolecule in Brain and Eye of Poikilothermic Vertebrates. Biomolecules. 2015 Apr 24;5(2):635-46. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.6466 mL	23.2331 mL	46.4662 mL	116.1656 mL
	5 mM	0.9293 mL	4.6466 mL	9.2932 mL	23.2331 mL
	10 mM	0.4647 mL	2.3233 mL	4.6466 mL	11.6166 mL
	15 mM	0.3098 mL	1.5489 mL	3.0977 mL	7.7444 mL
	20 mM	0.2323 mL	1.1617 mL	2.3233 mL	5.8083 mL
	25 mM	0.1859 mL	0.9293 mL	1.8586 mL	4.6466 mL
	30 mM	0.1549 mL	0.7744 mL	1.5489 mL	3.8722 mL
	40 mM	0.1162 mL	0.5808 mL	1.1617 mL	2.9041 mL
	50 mM	0.0929 mL	0.4647 mL	0.9293 mL	2.3233 mL
	60 mM	0.0774 mL	0.3872 mL	0.7744 mL	1.9361 mL
	80 mM	0.0581 mL	0.2904 mL	0.5808 mL	1.4521 mL
H₂O	100 mM	0.0465 mL	0.2323 mL	0.4647 mL	1.1617 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.