N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine (Synonyms: 5'-O-DMT-N4-Bz-dC)

Cat. No.: HY-W010706 Purity: 99.55%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine (5'-O-DMT-N4-Bz-dC) can be used for synthesis oligodeoxynucleotides containing a 3'-S-phosphorothiolate (3'-PS) linkage. N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine is an useful tool for probing enzyme-catalyzed cleavage processes in DNA.

For research use only. We do not sell to patients.

N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine Chemical Structure

CAS No. : 67219-55-0

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5 g	USD 51	Ask For Quote & Lead Time

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Purity & Documentation
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Description

Molecular Weight

633.69

Formula

C₃₇H₃₅N₃O₇

CAS No.

67219-55-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(N=C(NC(C1=CC=CC=C1)=O)C=C2)N2[C@H]3C[C@H](O)[C@@H](COC(C4=CC=C(OC)C=C4)(C5=CC=C(OC)C=C5)C6=CC=CC=C6)O3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (157.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5781 mL	7.8903 mL	15.7806 mL
5 mM	0.3156 mL	1.5781 mL	3.1561 mL
10 mM	0.1578 mL	0.7890 mL	1.5781 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.95 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (278 KB) SDS (418 KB)

COA (258 KB) HNMR (316 KB) RP-HPLC (190 KB)

Handling Instructions (2659 KB)

References

[1]. James W Gaynor, et al. Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage. Nat Protoc. 2007;2(12):3122-35. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5781 mL	7.8903 mL	15.7806 mL	39.4515 mL
	5 mM	0.3156 mL	1.5781 mL	3.1561 mL	7.8903 mL
	10 mM	0.1578 mL	0.7890 mL	1.5781 mL	3.9451 mL
	15 mM	0.1052 mL	0.5260 mL	1.0520 mL	2.6301 mL
	20 mM	0.0789 mL	0.3945 mL	0.7890 mL	1.9726 mL
	25 mM	0.0631 mL	0.3156 mL	0.6312 mL	1.5781 mL
	30 mM	0.0526 mL	0.2630 mL	0.5260 mL	1.3150 mL
	40 mM	0.0395 mL	0.1973 mL	0.3945 mL	0.9863 mL
	50 mM	0.0316 mL	0.1578 mL	0.3156 mL	0.7890 mL
	60 mM	0.0263 mL	0.1315 mL	0.2630 mL	0.6575 mL
	80 mM	0.0197 mL	0.0986 mL	0.1973 mL	0.4931 mL
	100 mM	0.0158 mL	0.0789 mL	0.1578 mL	0.3945 mL

N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine67219-55-05'-O-DMT-N4-Bz-dCNucleoside Antimetabolite/AnalogInhibitorinhibitorinhibit

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