Neocryptotanshinone

Name: Neocryptotanshinone
Brand: MedChemExpress
SKU: HY-119720
Rating: 4.5 (1 reviews)

Cat. No.: HY-119720 Purity: 98.82%: FAQs
Data Sheet SDS COA Handling Instructions

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Neocryptotanshinone, a fatty diterpenoids from Salvia Miltiorrhiza, inhibits lipopolysaccharide-induced inflammation by suppression of NF-κB and iNOS signaling pathways.

For research use only. We do not sell to patients.

Neocryptotanshinone Chemical Structure

CAS No. : 109664-02-0

Size	Price	Stock	Quantity
5 mg	USD 375	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 665	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

Neocryptotanshinone, a fatty diterpenoids from Salvia Miltiorrhiza, inhibits lipopolysaccharide-induced inflammation by suppression of NF-κB and iNOS signaling pathways^[1]^[2].

IC₅₀ & Target

iNOS

In Vitro

Neocryptotanshinone exhibits anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways^[1].
Neocryptotanshinone (10 μM and 20 μM, 24 h) inhibits LPS-induced iNOS protein expression in RAW264.7 cells^[1].
Neocryptotanshinone (20 μM, 24 h) shows no obvious cytotoxic effect towards murine RAW264.7 macrophages^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	RAW264.7 cells
Concentration:	5, 10 and 20 μM
Incubation Time:	24 hours
Result:	Inhibited LPS-induced cell viability in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	RAW264.7 cells
Concentration:	20 μM
Incubation Time:	24 hours
Result:	Inhibited LPS-induced activation of NF-κB pathway and down-regulated LPS-induced expression of p-NF-κB p65, p-IκBα and p-IKKβ.

In Vivo

Neocryptotanshinone causes reversals of decreased pain thresholds induced by MSU treatment after 30, 60, and 120 min S. miltiorrhiza Bunge extract treatment (contains single active components)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	MSU-induced pain model in male ICR mice (weighing 20-25 g)^[2]
Dosage:	10, 25, 50 or 100 mg/kg
Administration:	Oral gavage; for 30, 60, 120 min
Result:	Inhibited inflammatory symptoms and nociceptive behaviors in a dose-dependent manner.

Molecular Weight

314.38

Formula

C₁₉H₂₂O₄

CAS No.

109664-02-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C([C@@H](C)CO)=C(O)C(C2=C1C=CC3=C2CCCC3(C)C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (318.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1809 mL	15.9043 mL	31.8086 mL
5 mM	0.6362 mL	3.1809 mL	6.3617 mL
10 mM	0.3181 mL	1.5904 mL	3.1809 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.82%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (249 KB) HNMR (134 KB) RP-HPLC (169 KB) MS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Chuanhong Wu, et al. Neocryptotanshinone Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages by Suppression of NF-κB and iNOS Signaling Pathways. Acta Pharm Sin B. 2015 Jul;5(4):323-9. [Content Brief]

[2]. H C Lin, et al. Two New Fatty Diterpenoids From Salvia Miltiorrhiza. J Nat Prod. 2001 May;64(5):648-50. [Content Brief]

[3]. FENG Jinghui, et al. Effects of Salvia miltiorrhiza Bunge extract and its single components on monosodium urate-induced pain in vivo and lipopolysaccharide-induced inflammation in vitro. J Tradit Chin Med. 2021. 41(2): 219-226. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1809 mL	15.9043 mL	31.8086 mL	79.5216 mL
	5 mM	0.6362 mL	3.1809 mL	6.3617 mL	15.9043 mL
	10 mM	0.3181 mL	1.5904 mL	3.1809 mL	7.9522 mL
	15 mM	0.2121 mL	1.0603 mL	2.1206 mL	5.3014 mL
	20 mM	0.1590 mL	0.7952 mL	1.5904 mL	3.9761 mL
	25 mM	0.1272 mL	0.6362 mL	1.2723 mL	3.1809 mL
	30 mM	0.1060 mL	0.5301 mL	1.0603 mL	2.6507 mL
	40 mM	0.0795 mL	0.3976 mL	0.7952 mL	1.9880 mL
	50 mM	0.0636 mL	0.3181 mL	0.6362 mL	1.5904 mL
	60 mM	0.0530 mL	0.2651 mL	0.5301 mL	1.3254 mL
	80 mM	0.0398 mL	0.1988 mL	0.3976 mL	0.9940 mL
	100 mM	0.0318 mL	0.1590 mL	0.3181 mL	0.7952 mL