2. GPCR/G Protein Neuronal Signaling
  3. GPR55
  4. O-1602

O-1602 is an agonist of GPR55 (G protein-coupled receptor 55). O-1602 reduces the number and activation of hippocampal microglia induced by METH (methamphetamine). O-1602 decreases the expression levels of NLRP3 inflammasome proteins, including NLRP3, ASC and Caspase-1.

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O-1602 Chemical Structure

O-1602 Chemical Structure

CAS No. : 317321-41-8

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Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 132 In-stock
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10 mM * 1 mL in DMSO USD 132 In-stock
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1 mg USD 36 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of O-1602:

O-1602 Related Antibodies

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  • Purity & Documentation

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Description

O-1602 is an agonist of GPR55 (G protein-coupled receptor 55). O-1602 reduces the number and activation of hippocampal microglia induced by METH (methamphetamine). O-1602 decreases the expression levels of NLRP3 inflammasome proteins, including NLRP3, ASC and Caspase-1[1].

In Vitro

O-1602 (10 μM and 100 μM; 10 days) increases intracellular calcium levels, promotes lipid accumulation, and increases the expression of CEBP-α, a key regulator of adipocyte differentiation at 100 μM[2].
O-1602 (0 μM and 10 μM; 10 days) promotes the accumulation of lipid in 3T3-L1 adipocytes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

O-1602 Related Antibodies

Real Time qPCR[2]

Cell Line: 3T3-L1 cells
Concentration: 0.5 µg
Incubation Time: 6 h
Result: Decreased the expression of neuropeptide Y, proopiate melanocortin, and cocaine and amphetamine regulatory transcript (CART) mRNA.
In Vivo

O-1602 (10 mg/kg, ip; once daily for 14 consecutive days) decreases levels of serum corticosterone, TNF-α, IL-1β, and IL-6 [1].
O-1602 (10 mg/kg, ip; once daily for 14 consecutive days) increases hippocampal GPR55 protein expression [1].
O-1602 (10 mg/kg, ip; once daily for 14 consecutive days) significantly increases DCX expression in the DG [1].
O-1602 (10 mg/kg, ip; once daily for 14 consecutive days) significantly decreases the number of microglia in the hippocampus [1].
O-1602 (10 mg/kg, ip; once daily for 14 consecutive days) increases expression levels of NLRP3, ASC, and Caspase-1 in the hippocampus [1].
O-1602 (0.1 mg/kg,ip; subchronically infused for 7 days) decreases the percentage of fat utilization over total energy consumption and decreases metabolic use of lipids leading to elevated fat deposition rates[2].
O-1602 (0.04 and 0.4 μg/h/rat, ip; for 7 days) increases the the amount of fat mass with O-1602 at the dose of 0.4 μg/h/rat[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: the model of METH-induced anxiety- and depression-like behaviors [1]
Dosage: 10 mg/kg, once daily for 14 consecutive days
Administration: Intraperitoneal injection (i.p.)
Result: Increased both time spent in the center area of the open field test and time exploring the open arms in the elevated plus maze test. Reduced immobility time in the forced swim and tail suspension tests.
Animal Model: Adult male Sprague–Dawley rats (Harlam Iberica, Barcelona, Spain) (250–275 g, 10–12 weeks old)[2]
Dosage: 0.1, 0.5 and 1 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Did not modify food intake and body weight gain.Increased the fat mass.
Molecular Weight

258.36

Formula

C17H22O2
CAS No.
Appearance

Oil

Color

White to light yellow

SMILES

CC1=C([C@H]2[C@@H](CCC(C)=C2)C(C)=C)C(O)=CC(O)=C1
Shipping

Shipping with dry ice.
Storage

-80°C
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (387.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8706 mL 19.3528 mL 38.7057 mL
5 mM 0.7741 mL 3.8706 mL 7.7411 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months. When stored at -80°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months. When stored at -80°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8706 mL 19.3528 mL 38.7057 mL 96.7642 mL
5 mM 0.7741 mL 3.8706 mL 7.7411 mL 19.3528 mL
10 mM 0.3871 mL 1.9353 mL 3.8706 mL 9.6764 mL
15 mM 0.2580 mL 1.2902 mL 2.5804 mL 6.4509 mL
20 mM 0.1935 mL 0.9676 mL 1.9353 mL 4.8382 mL
25 mM 0.1548 mL 0.7741 mL 1.5482 mL 3.8706 mL
30 mM 0.1290 mL 0.6451 mL 1.2902 mL 3.2255 mL
40 mM 0.0968 mL 0.4838 mL 0.9676 mL 2.4191 mL
50 mM 0.0774 mL 0.3871 mL 0.7741 mL 1.9353 mL
60 mM 0.0645 mL 0.3225 mL 0.6451 mL 1.6127 mL
80 mM 0.0484 mL 0.2419 mL 0.4838 mL 1.2096 mL
100 mM 0.0387 mL 0.1935 mL 0.3871 mL 0.9676 mL
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O-1602 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

O-1602317321-41-8O1602O 1602GPR55G protein-coupled receptor 55GPR55 agonistDepressionHippocampal microgliaModel of METH-induced anxiety- and depression-like behaviorsadipose tissueInhibitorinhibitorinhibit

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