2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK Stem Cell/Wnt
  3. EGFR STAT
  4. OK2

OK2, a specific inhibitor of the CCN2/EGFR interaction, efficiently blocks CCN2/EGFR interaction through binding to the CT domain of CCN2. OK2 can be used for kidney fibrosis and chronic kidney disease research.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

OK2 Chemical Structure

OK2 Chemical Structure

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10 mg USD 250 In-stock
25 mg USD 480 In-stock
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Based on 1 publication(s) in Google Scholar

OK2 Related Antibodies

Top Publications Citing Use of Products

View All EGFR Isoform Specific Products:

View All Isoforms
EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

View All STAT Isoform Specific Products:

View All Isoforms
STAT3 STAT5 STAT6 STAT1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

OK2, a specific inhibitor of the CCN2/EGFR interaction, efficiently blocks CCN2/EGFR interaction through binding to the CT domain of CCN2. OK2 can be used for kidney fibrosis and chronic kidney disease research[1].

IC50 & Target

p-STAT3

 

In Vitro

OK2 (0-100 μM; 24 hours) inhibits EGFR activation, STAT3 phosphorylation, and ECM protein synthesis in HK-2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

OK2 Related Antibodies

Western Blot Analysis[1]

Cell Line: HK-2 cells
Concentration: 0, 4, 20, 100 μM
Incubation Time: 24 hours
Result: Blocked thevastmajority ofEGFR activation in therenalfibrosis cell model, thereby preventing EGFRmediated STAT3 phosphorylation andECMprotein synthesis.
Inhibited EGFR-mediated ECM protein synthesis in a dose dependent manner and exhibited a significant blocking effect beginning at 20 μM.
In Vivo

OK2 (50 μg/kg, i.p., for 14 days) blocks CCN2-induced EGFR/STAT3 activation and impede kidney fibrosis in the unilateral ureteric obstruction (UUO) mice model[1].
OK2 (1 mg/kg, s.c.) shows a T1/2 of 0.9 h and Cmax of 1057.8 ng/mL in male Sprague Dawley rats[1].
Pharmacokinetic parameters for OK2 in male Sprague Dawley rats [1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Route Dose (mg/kg) Cmax (ng/mL) Tmax (h) T1/2 (h) AUC0-t (h•ng/mL)
s.c. 1 1057.8 1.0 0.9 3032.0
Animal Model: Unilateral ureteric obstruction (UUO)-induced renal fibrosis [1]
Dosage: 50 μg/kg
Administration: Intraperitoneal injection (i.p.) for 14 days
Result: Alleviated renal tubular injury and inflammatory infiltration, as well as suppressed collagen formation in the left kidney.
Inhibited the phosphorylation of EGFR and STAT3 and reduced the vast majority of fibronectin and type I collagen synthesis.
Animal Model: Male Sprague Dawley rats(Pharmacokinetic assay)[1]
Dosage: 1 mg/kg
Administration: Subcutaneous injection (s.c.)
Result: Showed a T1/2 of 0.9 h and Cmax of 1057.8 ng/mL in male Sprague Dawley rats[1].
Molecular Weight

907.03

Formula

C42H62N14O9
Appearance

Solid

Color

White to off-white

Sequence

cyclo-(Thr-Lys-His-Arg-Pro-Trp-Thr)
Sequence Shortening

cyclo-(TKHRPWT)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (110.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1025 mL 5.5125 mL 11.0250 mL
5 mM 0.2205 mL 1.1025 mL 2.2050 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1025 mL 5.5125 mL 11.0250 mL 27.5625 mL
5 mM 0.2205 mL 1.1025 mL 2.2050 mL 5.5125 mL
10 mM 0.1102 mL 0.5512 mL 1.1025 mL 2.7562 mL
15 mM 0.0735 mL 0.3675 mL 0.7350 mL 1.8375 mL
20 mM 0.0551 mL 0.2756 mL 0.5512 mL 1.3781 mL
25 mM 0.0441 mL 0.2205 mL 0.4410 mL 1.1025 mL
30 mM 0.0367 mL 0.1837 mL 0.3675 mL 0.9187 mL
40 mM 0.0276 mL 0.1378 mL 0.2756 mL 0.6891 mL
50 mM 0.0220 mL 0.1102 mL 0.2205 mL 0.5512 mL
60 mM 0.0184 mL 0.0919 mL 0.1837 mL 0.4594 mL
80 mM 0.0138 mL 0.0689 mL 0.1378 mL 0.3445 mL
100 mM 0.0110 mL 0.0551 mL 0.1102 mL 0.2756 mL
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OK2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OK2OK 2OK-2EGFRSTATEpidermal growth factor receptorErbB-1HER1Kidney FibrosisChronic Kidney DiseaseHK-2STAT3CCN2/EGFR Interaction InhibitorInhibitorinhibitorinhibit

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