Oxamic acid (Synonyms: Oxamidic acid)

Cat. No.: HY-W013032 Purity: ≥98.0%: FAQs
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Oxamic acid is a lactate dehydrogenase-A (LDH-A) inhibitor. Oxamic acid shows anti-tumor activity, and anti-proliferative activity against cancer cells, and can induce apoptosis.

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Oxamic acid Chemical Structure

CAS No. : 471-47-6

Size	Price	Stock
100 mg	USD 30	Ask For Quote & Lead Time
500 mg	USD 35	Ask For Quote & Lead Time

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Other Forms of Oxamic acid:

Oxamic acid sodium Get quote

7 Publications Citing Use of MCE Oxamic acid

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Purity & Documentation
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Description

Oxamic acid is a lactate dehydrogenase-A (LDH-A) inhibitor. Oxamic acid shows anti-tumor activity, and anti-proliferative activity against cancer cells, and can induce apoptosis^[1]^[2]^[3].

In Vitro

Oxamic acid suppresses the proliferation, migration and invasion of both A2780 and SKOV3 cells^[1].
Oxamic acid (10 μM; 24-72 h) inhibits cell proliferation in a dose- and time-dependent manner in both NPC cancer cells^[2].
Oxamic acid (0-100 mM; 24 h) induces cell cycle arrest in the G2/M phase in CNE-1 and CNE-2 cells^[2].
Oxamic acid (0-100 mM; 48 h) induces apoptosis via caspase-3 activation and the mitochondrial pathway in NPC cells^[2].
Oxamic acid (0-100 mM; 24 h) increases ROS levels in NPC cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	CNE-1 and CNE-2 cells
Concentration:	10 μM
Incubation Time:	24-72 hours
Result:	Showed IC₅₀s of 74.6, 32.4 and 17.8 mM and 62.3, 44.5, 31.6 mM at 24, 48 and 72 h in the CNE-1 and CNE-2 cancer cells, respectively.

Apoptosis Analysis^[2]

Cell Line:	CNE-1 and CNE-2 cells
Concentration:	0, 20, 50 and 100 mM
Incubation Time:	48 hours
Result:	Showed the increasement of early and late apoptotic cells in a dose-dependent manner. Increased the expression of pro-apoptotic Bax and cleaved-caspase-3, while reduced the anti-apoptotic signals of Bcl-2 and pro-caspase-3.

Cell Cycle Analysis^[2]

Cell Line:	CNE-1 and CNE-2 cells
Concentration:	0, 20, 50 and 100 mM
Incubation Time:	24 hours
Result:	Showed a dose-dependent increase in the numbers of CNE-1 and CNE-2 cells in the G2/M phase.

In Vivo

Oxamic acid (intraperitoneal injection; 750 mg/kg; once daily; 3 w) treatment improves the efficacy of tumor inhibition in vivo when combined with irradiation treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Balb/c nude mice injected with CNE-1 cells^[2]
Dosage:	750 mg/kg
Administration:	Intraperitoneal injection; 750 mg/kg; once daily; 3 weeks
Result:	Inhibited the tumor growth when compared to either oxamate alone or irradiation alone.

Molecular Weight

89.05

Formula

C₂H₃NO₃

CAS No.

471-47-6

Appearance

Solid

Color

White to off-white

SMILES

NC(C(O)=O)=O

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (1122.96 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	11.2296 mL	56.1482 mL	112.2965 mL
5 mM	2.2459 mL	11.2296 mL	22.4593 mL
10 mM	1.1230 mL	5.6148 mL	11.2296 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (276 KB) SDS (760 KB)

COA (245 KB) RP-HPLC (293 KB)

Handling Instructions (2659 KB)

References

[1]. Xiang J, et al. LDH-A inhibitors as remedies to enhance the anticancer effects of PARP inhibitors in ovarian cancer cells. Aging (Albany NY). 2021 Dec 16;13(24):25920-25930. [Content Brief]

[2]. Zhai X, et al. Inhibition of LDH-A by oxamate induces G2/M arrest, apoptosis and increases radiosensitivity in nasopharyngeal carcinoma cells. Oncol Rep. 2013 Dec;30(6):2983-91. [Content Brief]

[3]. Muramatsu H, et al. Targeting lactate dehydrogenase‑A promotes docetaxel‑induced cytotoxicity predominantly in castration‑resistant prostate cancer cells. Oncol Rep. 2019 Jul;42(1):224-230. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	11.2296 mL	56.1482 mL	112.2965 mL	280.7411 mL
	5 mM	2.2459 mL	11.2296 mL	22.4593 mL	56.1482 mL
	10 mM	1.1230 mL	5.6148 mL	11.2296 mL	28.0741 mL
	15 mM	0.7486 mL	3.7432 mL	7.4864 mL	18.7161 mL
	20 mM	0.5615 mL	2.8074 mL	5.6148 mL	14.0371 mL
	25 mM	0.4492 mL	2.2459 mL	4.4919 mL	11.2296 mL
	30 mM	0.3743 mL	1.8716 mL	3.7432 mL	9.3580 mL
	40 mM	0.2807 mL	1.4037 mL	2.8074 mL	7.0185 mL
	50 mM	0.2246 mL	1.1230 mL	2.2459 mL	5.6148 mL
	60 mM	0.1872 mL	0.9358 mL	1.8716 mL	4.6790 mL
	80 mM	0.1404 mL	0.7019 mL	1.4037 mL	3.5093 mL
	100 mM	0.1123 mL	0.5615 mL	1.1230 mL	2.8074 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Oxamic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oxamic acid471-47-6Oxamidic acidLactate DehydrogenaseApoptosisLDHanti-tumor activityanti-proliferative activityinduce apoptosisCNE-1CNE-2A2780SKOV3Inhibitorinhibitorinhibit

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