p-Tolylmaleimide

Cat. No.: HY-129592 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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p-Tolylmaleimide (compound 9) is a naphthalimide derivative that has cytotoxic effects on cancer cells. p-Tolylmaleimide can arrest the cell cycle of human acute myeloid leukemia cells K562 in the sub-G0/G1 phase and induce apoptosis.

For research use only. We do not sell to patients.

p-Tolylmaleimide Chemical Structure

CAS No. : 1631-28-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 360	Get quote	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	GI₅₀	> 10 μg/mL Compound: 36	Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
NCI-H460	GI₅₀	> 10 μg/mL Compound: 36	Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
SF-268	GI₅₀	> 10 μg/mL Compound: 36	Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]

In Vitro

p-Tolylmaleimide inhibits human acute myeloid leukemia cell K562 with IC₅₀s of 24.99 μM (24 h), 6.61 (48 h), and 12.10 (72 h) respectively. p-Tolylmaleimide for inhibits human acute myeloid leukemia cell Jurkat with IC₅₀s of 27.16 μM (24 h), 15.51 (48 h), and 3.94 (72 h) respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

187.19

Formula

C₁₁H₉NO₂

CAS No.

1631-28-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C=C1)N(C2=CC=C(C)C=C2)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (534.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3422 mL	26.7108 mL	53.4217 mL
5 mM	1.0684 mL	5.3422 mL	10.6843 mL
10 mM	0.5342 mL	2.6711 mL	5.3422 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (276 KB) SDS (555 KB)

COA (256 KB) HNMR (128 KB)

Handling Instructions (2659 KB)

References

[1]. Machado KE, et al. Apoptotic events induced by maleimides on human acute leukemia cell lines. Chem Res Toxicol. 2013 Dec 16;26(12):1904-16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3422 mL	26.7108 mL	53.4217 mL	133.5541 mL
	5 mM	1.0684 mL	5.3422 mL	10.6843 mL	26.7108 mL
	10 mM	0.5342 mL	2.6711 mL	5.3422 mL	13.3554 mL
	15 mM	0.3561 mL	1.7807 mL	3.5614 mL	8.9036 mL
	20 mM	0.2671 mL	1.3355 mL	2.6711 mL	6.6777 mL
	25 mM	0.2137 mL	1.0684 mL	2.1369 mL	5.3422 mL
	30 mM	0.1781 mL	0.8904 mL	1.7807 mL	4.4518 mL
	40 mM	0.1336 mL	0.6678 mL	1.3355 mL	3.3389 mL
	50 mM	0.1068 mL	0.5342 mL	1.0684 mL	2.6711 mL
	60 mM	0.0890 mL	0.4452 mL	0.8904 mL	2.2259 mL
	80 mM	0.0668 mL	0.3339 mL	0.6678 mL	1.6694 mL
	100 mM	0.0534 mL	0.2671 mL	0.5342 mL	1.3355 mL