Pennogenin 3-O-beta-chacotrioside

Name: Pennogenin 3-O-beta-chacotrioside
Brand: MedChemExpress
SKU: HY-N4180
Rating: 4.5 (1 reviews)

Cat. No.: HY-N4180 Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pennogenin 3-O-beta-chacotrioside is an active component isolated from Paris polyphylla, modulates autophagy via increasing the expressions of autophagy-related proteins LC3 and Beclin-1. Anti-colorectal cancer activity.

For research use only. We do not sell to patients.

Pennogenin 3-O-beta-chacotrioside Chemical Structure

CAS No. : 55916-52-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 262	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 262	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 158	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 269	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 457	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Autophagy^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	4.2 μg/mL Compound: 6	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 11000027]

Molecular Weight

885.04

Formula

C₄₅H₇₂O₁₇

CAS No.

55916-52-4

Appearance

Solid

Color

White to off-white

SMILES

O[C@@]12[C@@]3([C@@]([C@@]4([H])[C@]([C@@]5(C(C[C@@H](O[C@@]6([H])[C@@H]([C@H]([C@H](O[C@@]7([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O7)O)O)[C@@H](CO)O6)O)O[C@@]8([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O8)O)O)CC5)=CC4)C)([H])CC3)([H])C[C@]1([H])O[C@]9(CC[C@@H](C)CO9)[C@H]2C)C

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (112.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1299 mL	5.6495 mL	11.2989 mL
5 mM	0.2260 mL	1.1299 mL	2.2598 mL
10 mM	0.1130 mL	0.5649 mL	1.1299 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (255 KB) HNMR (413 KB) RP-HPLC (74 KB)

Handling Instructions (2659 KB)

References

[1]. Lin LT, et al. Paris Polyphylla Inhibits Colorectal Cancer Cells via Inducing Autophagy and Enhancing the Efficacy of Chemotherapeutic Drug Doxorubicin. Molecules. 2019 Jun 3;24(11). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1299 mL	5.6495 mL	11.2989 mL	28.2473 mL
	5 mM	0.2260 mL	1.1299 mL	2.2598 mL	5.6495 mL
	10 mM	0.1130 mL	0.5649 mL	1.1299 mL	2.8247 mL
	15 mM	0.0753 mL	0.3766 mL	0.7533 mL	1.8832 mL
	20 mM	0.0565 mL	0.2825 mL	0.5649 mL	1.4124 mL
	25 mM	0.0452 mL	0.2260 mL	0.4520 mL	1.1299 mL
	30 mM	0.0377 mL	0.1883 mL	0.3766 mL	0.9416 mL
	40 mM	0.0282 mL	0.1412 mL	0.2825 mL	0.7062 mL
	50 mM	0.0226 mL	0.1130 mL	0.2260 mL	0.5649 mL
	60 mM	0.0188 mL	0.0942 mL	0.1883 mL	0.4708 mL
	80 mM	0.0141 mL	0.0706 mL	0.1412 mL	0.3531 mL
	100 mM	0.0113 mL	0.0565 mL	0.1130 mL	0.2825 mL