1. Metabolic Enzyme/Protease
  2. Ser/Thr Protease
  3. Phe-Pro-Ala-pNA

Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. Phe-Pro-Ala-pNA can be used to test tripeptidyl peptidase activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Phe-Pro-Ala-pNA Chemical Structure

Phe-Pro-Ala-pNA Chemical Structure

CAS No. : 201738-99-0

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1 mg USD 60 In-stock
5 mg USD 150 In-stock
10 mg USD 250 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. Phe-Pro-Ala-pNA can be used to test tripeptidyl peptidase activity[1].

Molecular Weight

453.49

Formula

C23H27N5O5

CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Phe-Pro-{Ala-pNA}

Sequence Shortening

FP-{Ala-pNA}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 250 mg/mL (551.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2051 mL 11.0256 mL 22.0512 mL
5 mM 0.4410 mL 2.2051 mL 4.4102 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2051 mL 11.0256 mL 22.0512 mL 55.1280 mL
5 mM 0.4410 mL 2.2051 mL 4.4102 mL 11.0256 mL
10 mM 0.2205 mL 1.1026 mL 2.2051 mL 5.5128 mL
15 mM 0.1470 mL 0.7350 mL 1.4701 mL 3.6752 mL
20 mM 0.1103 mL 0.5513 mL 1.1026 mL 2.7564 mL
25 mM 0.0882 mL 0.4410 mL 0.8820 mL 2.2051 mL
30 mM 0.0735 mL 0.3675 mL 0.7350 mL 1.8376 mL
40 mM 0.0551 mL 0.2756 mL 0.5513 mL 1.3782 mL
50 mM 0.0441 mL 0.2205 mL 0.4410 mL 1.1026 mL
60 mM 0.0368 mL 0.1838 mL 0.3675 mL 0.9188 mL
80 mM 0.0276 mL 0.1378 mL 0.2756 mL 0.6891 mL
100 mM 0.0221 mL 0.1103 mL 0.2205 mL 0.5513 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Phe-Pro-Ala-pNA
Cat. No.:
HY-P4470
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