2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. COX Lipoxygenase
  4. Phenidone

Phenidone, an orally active dual inhibitor of cyclooxygenase (COX) and lipoxygenase (LOX), ameliorates rat paralysis in experimental autoimmune encephalomyelitis. Phenidone is a potent hypotensive agent in the spontaneously hypertensive rat. Phenidone is used as a photographic developer.

For research use only. We do not sell to patients.

Phenidone Chemical Structure

Phenidone Chemical Structure

CAS No. : 92-43-3

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
500 mg USD 50 In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Phenidone Related Antibodies

Top Publications Citing Use of Products

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

View All Lipoxygenase Isoform Specific Products:

View All Isoforms
5-LOX 12-LOX 15-LOX

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Phenidone, an orally active dual inhibitor of cyclooxygenase (COX) and lipoxygenase (LOX), ameliorates rat paralysis in experimental autoimmune encephalomyelitis. Phenidone is a potent hypotensive agent in the spontaneously hypertensive rat[1][2]. Phenidone is used as a photographic developer[3].

IC50 & Target[1]

COX

 

LOX

 

Cellular Effect
Cell Line Type Value Description References
RBL-1 IC50
0.57 μM
Compound: Phenidone
Ability to inhibit 5-lipoxygenase by using a crude preparation of the cytosolic enzyme from the rat basophilic leukemia (RBL-1) cell line
Ability to inhibit 5-lipoxygenase by using a crude preparation of the cytosolic enzyme from the rat basophilic leukemia (RBL-1) cell line
[PMID: 2104936]
RBL-1 IC50
1.9 μM
Compound: 1 phenidone
Inhibition of 5-lipoxygenase catalysis in rat basophilic leukemia (RBL) cells by measuring 5-HETE product formation
Inhibition of 5-lipoxygenase catalysis in rat basophilic leukemia (RBL) cells by measuring 5-HETE product formation
[PMID: 8831760]
In Vitro

Phenidone significantly inhibits the increases in COX-1/-2 and 5-LOX in the spinal cords of rats with experimental autoimmune encephalomyelitis (EAE)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phenidone Related Antibodies
In Vivo

Phenidone inhibits the formation of vascular lipoxygenase products and to reduce blood pressure in the AII-dependent renovascular hypertensive rat[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

162.19

Formula

C9H10N2O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1NN(C2=CC=CC=C2)CC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (616.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 3.33 mg/mL (20.53 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1656 mL 30.8280 mL 61.6561 mL
5 mM 1.2331 mL 6.1656 mL 12.3312 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.1656 mL 30.8280 mL 61.6561 mL 154.1402 mL
5 mM 1.2331 mL 6.1656 mL 12.3312 mL 30.8280 mL
10 mM 0.6166 mL 3.0828 mL 6.1656 mL 15.4140 mL
15 mM 0.4110 mL 2.0552 mL 4.1104 mL 10.2760 mL
20 mM 0.3083 mL 1.5414 mL 3.0828 mL 7.7070 mL
DMSO 25 mM 0.2466 mL 1.2331 mL 2.4662 mL 6.1656 mL
30 mM 0.2055 mL 1.0276 mL 2.0552 mL 5.1380 mL
40 mM 0.1541 mL 0.7707 mL 1.5414 mL 3.8535 mL
50 mM 0.1233 mL 0.6166 mL 1.2331 mL 3.0828 mL
60 mM 0.1028 mL 0.5138 mL 1.0276 mL 2.5690 mL
80 mM 0.0771 mL 0.3854 mL 0.7707 mL 1.9268 mL
100 mM 0.0617 mL 0.3083 mL 0.6166 mL 1.5414 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Phenidone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenidone92-43-3COXLipoxygenaseCyclooxygenaseLOXautoimmunehypertensivebloodpressureoralphotographicdeveloperInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Phenidone
Cat. No.:
HY-W010144
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (536 KB) Français - FR (536 KB) Deutsch - DE (536 KB) Norwegian - NO (536 KB) Español - ES (536 KB) Swedish - SV (536 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.