2. Apoptosis
  3. MDM-2/p53
  4. Pifithrin-α, p-Nitro, Cyclic

Pifithrin-α, p-Nitro, Cyclic  (Synonyms: PFN-α)

Cat. No.: HY-123076 Purity: 99.73%
COA Handling Instructions

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Pifithrin-α, p-Nitro, Cyclic (PFN-α) is cell-permeable and active-form p53 inhibitor. Pifithrin-α, p-Nitro, Cyclic is one order magnitude more active than Pifithrin-α in protecting cortical neurons exposed to Etoposide (ED50=30 nM). Pifithrin-α, p-Nitro, Cyclic behaves as a p53 posttranscriptional activity inhibitor. Pifithrin-α, p-Nitro, Cyclic do not prevent p53 phosphorylation on the S15 residue.

For research use only. We do not sell to patients.

Pifithrin-α, p-Nitro, Cyclic Chemical Structure

Pifithrin-α, p-Nitro, Cyclic Chemical Structure

CAS No. : 60477-38-5

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Based on 4 publication(s) in Google Scholar

Pifithrin-α, p-Nitro, Cyclic Related Antibodies

Top Publications Citing Use of Products

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  • Purity & Documentation

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Description

Pifithrin-α, p-Nitro, Cyclic (PFN-α) is cell-permeable and active-form p53 inhibitor. Pifithrin-α, p-Nitro, Cyclic is one order magnitude more active than Pifithrin-α in protecting cortical neurons exposed to Etoposide (ED50=30 nM). Pifithrin-α, p-Nitro, Cyclic behaves as a p53 posttranscriptional activity inhibitor. Pifithrin-α, p-Nitro, Cyclic do not prevent p53 phosphorylation on the S15 residue[1][2].

In Vitro

Pifithrin-α, p-Nitro, Cyclic (PFN-α) efficiently blocks p53-triggered cell death and p21/WAF1 expression in cortical neurons exposed to etoposide at concentrations one order magnitude lower than that in PFT-α[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pifithrin-α, p-Nitro, Cyclic Related Antibodies
In Vivo

Intraocular injection of pifithrin-α slightly increases survival of retinal ganglion cell (RGC) at the concentration of 6 μM but not at 0.06 μM. Pifithrin-α, p-Nitro, Cyclic (PFN-α) is not effective in vivo, even at 6 μM[1].
Pifithrin-α, p-Nitro, Cyclic shows the half-life (t1/2) of 6 hours (when incubated in biological conditions)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

299.35

Formula

C15H13N3O2S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+]([O-])C1=CC=C(C2=CN3C(SC4=C3CCCC4)=N2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 2 years; -20°C, 1 year (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMF : 12.5 mg/mL (41.76 mM; Need ultrasonic)

DMSO : 5 mg/mL (16.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3406 mL 16.7029 mL 33.4057 mL
5 mM 0.6681 mL 3.3406 mL 6.6811 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.73%

COA (250 KB) HNMR (253 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / DMF 1 mM 3.3406 mL 16.7029 mL 33.4057 mL 83.5143 mL
5 mM 0.6681 mL 3.3406 mL 6.6811 mL 16.7029 mL
10 mM 0.3341 mL 1.6703 mL 3.3406 mL 8.3514 mL
15 mM 0.2227 mL 1.1135 mL 2.2270 mL 5.5676 mL
DMF 20 mM 0.1670 mL 0.8351 mL 1.6703 mL 4.1757 mL
25 mM 0.1336 mL 0.6681 mL 1.3362 mL 3.3406 mL
30 mM 0.1114 mL 0.5568 mL 1.1135 mL 2.7838 mL
40 mM 0.0835 mL 0.4176 mL 0.8351 mL 2.0879 mL
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Pifithrin-α, p-Nitro, Cyclic Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pifithrin-α, p-Nitro, Cyclic60477-38-5PFN-αMDM-2/p53celldeathneuronsp53-triggeredcorticalp21WAF1expressionposttranscriptionalInhibitorinhibitorinhibit

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