Podophyllotoxin glucoside

Name: Podophyllotoxin glucoside
Brand: MedChemExpress
SKU: HY-N1977
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1977 Purity: 98.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Podophyllotoxin glucoside is a podophyllotoxin derivative, has anti-tumor effects.

For research use only. We do not sell to patients.

Podophyllotoxin glucoside Chemical Structure

CAS No. : 16481-54-2

Size	Price	Stock	Quantity
1 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Podophyllotoxin glucoside is a podophyllotoxin derivative, has anti-tumor effects^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
GLC4 cell line	IC₅₀	10 μM Compound: 19	Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on continuous incubation Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on continuous incubation	[PMID: 7783142]
GLC4 cell line	IC₅₀	58 μM Compound: 19	Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on 2 hr incubation Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on 2 hr incubation	[PMID: 7783142]
HeLa	ED₅₀	21.8 μg/mL Compound: 6	Cytotoxicity against human HeLa cells after 4 days by trypan blue assay Cytotoxicity against human HeLa cells after 4 days by trypan blue assay	[PMID: 1602298]
HeLa	IC₅₀	6.65 μM Compound: 15	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 21570846]
KB	IC₅₀	9.25 μM Compound: 15	Cytotoxicity against human KB cells after 72 hrs by MTT assay Cytotoxicity against human KB cells after 72 hrs by MTT assay	[PMID: 21570846]

Molecular Weight

576.55

Formula

C₂₈H₃₂O₁₃

CAS No.

16481-54-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@]([C@]2([H])CO1)([H])[C@@](C(C=C3OC)=CC(OC)=C3OC)([H])C4=CC(OCO5)=C5C=C4[C@@H]2O[C@@H]([C@@H]([C@H]6O)O)O[C@@H]([C@H]6O)CO

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (86.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7345 mL	8.6723 mL	17.3445 mL
5 mM	0.3469 mL	1.7345 mL	3.4689 mL
10 mM	0.1734 mL	0.8672 mL	1.7345 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.17 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.17 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.17 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (261 KB) HNMR (242 KB) CNMR (104 KB) RP-HPLC (181 KB) MS (117 KB)

Handling Instructions (2659 KB)

References

[1]. K R Hande, et al. Etoposide: four decades of development of a topoisomerase II inhibitor. Eur J Cancer [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7345 mL	8.6723 mL	17.3445 mL	43.3614 mL
	5 mM	0.3469 mL	1.7345 mL	3.4689 mL	8.6723 mL
	10 mM	0.1734 mL	0.8672 mL	1.7345 mL	4.3361 mL
	15 mM	0.1156 mL	0.5782 mL	1.1563 mL	2.8908 mL
	20 mM	0.0867 mL	0.4336 mL	0.8672 mL	2.1681 mL
	25 mM	0.0694 mL	0.3469 mL	0.6938 mL	1.7345 mL
	30 mM	0.0578 mL	0.2891 mL	0.5782 mL	1.4454 mL
	40 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0840 mL
	50 mM	0.0347 mL	0.1734 mL	0.3469 mL	0.8672 mL
	60 mM	0.0289 mL	0.1445 mL	0.2891 mL	0.7227 mL
	80 mM	0.0217 mL	0.1084 mL	0.2168 mL	0.5420 mL