PPACK II diTFA

Name: PPACK II diTFA
Brand: MedChemExpress
SKU: HY-122543
Rating: 4.5 (1 reviews)

Cat. No.: HY-122543 Purity: 95.04%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PPACK II diTFA is an irreversible and specific glandular and plasma kallikreins inhibitor.

For research use only. We do not sell to patients.

PPACK II diTFA Chemical Structure

CAS No. : 649748-23-2

Size	Price	Stock	Quantity
5 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of PPACK II diTFA:

PPACK II Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Kallikrein Isoform Specific Products:

View All Isoforms

KLK1 KLK5 KLK8 KLK14 KLKB1

Biological Activity
Purity & Documentation
References
Customer Review

Description

PPACK II diTFA is an irreversible and specific glandular and plasma kallikreins inhibitor^[1].

In Vitro

PPACK II (0.62-50 μg/mL) diTFA significantly inhibits B-type natriuretic peptide (BNP) proteolysis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

729.07

Formula

C₂₉H₃₅ClF₆N₆O₇

CAS No.

649748-23-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C(F)(F)F.O=C(O)C(F)(F)F.N=C(N)NCCC[C@@H](C(CCl)=O)NC([C@@H](NC([C@H](N)CC1=CC=CC=C1)=O)CC2=CC=CC=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (342.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3716 mL	6.8581 mL	13.7161 mL
5 mM	0.2743 mL	1.3716 mL	2.7432 mL
10 mM	0.1372 mL	0.6858 mL	1.3716 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.04%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (252 KB) HNMR (329 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Kentaro Takayama, et al. Identification of a degrading enzyme in human serum that hydrolyzes a C-terminal core sequence of neuromedin U. Biopolymers. 2016 Nov 4;106(4):440-5. [Content Brief]

[2]. Alexander Belenky, et al. The effect of class-specific protease inhibitors on the stabilization of B-type natriuretic peptide in human plasma. Clin Chim Acta. 2004 Feb;340(1-2):163-72. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3716 mL	6.8581 mL	13.7161 mL	34.2903 mL
	5 mM	0.2743 mL	1.3716 mL	2.7432 mL	6.8581 mL
	10 mM	0.1372 mL	0.6858 mL	1.3716 mL	3.4290 mL
	15 mM	0.0914 mL	0.4572 mL	0.9144 mL	2.2860 mL
	20 mM	0.0686 mL	0.3429 mL	0.6858 mL	1.7145 mL
	25 mM	0.0549 mL	0.2743 mL	0.5486 mL	1.3716 mL
	30 mM	0.0457 mL	0.2286 mL	0.4572 mL	1.1430 mL
	40 mM	0.0343 mL	0.1715 mL	0.3429 mL	0.8573 mL
	50 mM	0.0274 mL	0.1372 mL	0.2743 mL	0.6858 mL
	60 mM	0.0229 mL	0.1143 mL	0.2286 mL	0.5715 mL
	80 mM	0.0171 mL	0.0857 mL	0.1715 mL	0.4286 mL
	100 mM	0.0137 mL	0.0686 mL	0.1372 mL	0.3429 mL

PPACK II diTFA Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PPACK II diTFA649748-23-2KallikreinKLKKallikrein-related peptidasekallikrein inhibitorB-type natriuretic peptideInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

PPACK II diTFA

Customer Review

Other Forms of PPACK II diTFA:

PPACK II diTFA Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Kallikrein Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

PPACK II diTFA Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

PPACK II diTFA Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report