2. Anti-infection Vitamin D Related/Nuclear Receptor Immunology/Inflammation
  3. Fungal Estrogen Receptor/ERR Androgen Receptor Aryl Hydrocarbon Receptor
  4. Prochloraz

Prochloraz  (Synonyms: BTS 40542)

Cat. No.: HY-B0845 Purity: 99.77%
COA Handling Instructions

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Prochloraz is an imidazole antifungal. Prochloraz is as an estrogen receptor (ER) and androgen receptor (AR) antagonist and an aromatase inhibitor with IC50 values of 25 μM, 4 μM and 0.3 μM, respectively. Prochloraz is able to activate the aryl hydrocarbon receptor (AhR) having an EC50 of 1 μM.

For research use only. We do not sell to patients.

Prochloraz Chemical Structure

Prochloraz Chemical Structure

CAS No. : 67747-09-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Prochloraz:

Prochloraz Related Antibodies

Top Publications Citing Use of Products

View All Estrogen Receptor/ERR Isoform Specific Products:

View All Isoforms
Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Prochloraz is an imidazole antifungal. Prochloraz is as an estrogen receptor (ER) and androgen receptor (AR) antagonist and an aromatase inhibitor with IC50 values of 25 μM, 4 μM and 0.3 μM, respectively. Prochloraz is able to activate the aryl hydrocarbon receptor (AhR) having an EC50 of 1 μM[1][2].

IC50 & Target

IC50: 25 μM (Estrogen receptor), 4 μM (Androgen receptor) and 0.3 μM (Aromatase)[2]
EC50: 1 μM (Aryl hydrocarbon receptor (AhR))[2]
In Vitro

Prochloraz has a strong inhibition of the microsomal cytochrome P-450 dependent 14α-demethylation of lanosterol during ergosterol biosynthesis, which leads to membrane disruption and eventually to cell death[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Prochloraz Related Antibodies
In Vivo

In vivo Prochloraz acts as an antiandrogen in the Hershberger assay by reducing weights of reproductive organs, affecting androgen-regulated gene expressions in the prostate and increasing luteinizing hormone levels. In order to investigate the developmental effects of Prochloraz, pregnant Wistar dams were dosed perinatally with 30 mg/kg Prochloraz. Results show that Prochloraz significantly reduces plasma and testicular testosterone levels in gestational day 21 male foetuses, whereas testicular progesterone was increased. Gestational length is increased by Prochloraz[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

376.67

Formula

C15H16Cl3N3O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C=CN=C1)N(CCC)CCOC2=C(Cl)C=C(Cl)C=C2Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (663.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6548 mL 13.2742 mL 26.5484 mL
5 mM 0.5310 mL 2.6548 mL 5.3097 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6548 mL 13.2742 mL 26.5484 mL 66.3711 mL
5 mM 0.5310 mL 2.6548 mL 5.3097 mL 13.2742 mL
10 mM 0.2655 mL 1.3274 mL 2.6548 mL 6.6371 mL
15 mM 0.1770 mL 0.8849 mL 1.7699 mL 4.4247 mL
20 mM 0.1327 mL 0.6637 mL 1.3274 mL 3.3186 mL
25 mM 0.1062 mL 0.5310 mL 1.0619 mL 2.6548 mL
30 mM 0.0885 mL 0.4425 mL 0.8849 mL 2.2124 mL
40 mM 0.0664 mL 0.3319 mL 0.6637 mL 1.6593 mL
50 mM 0.0531 mL 0.2655 mL 0.5310 mL 1.3274 mL
60 mM 0.0442 mL 0.2212 mL 0.4425 mL 1.1062 mL
80 mM 0.0332 mL 0.1659 mL 0.3319 mL 0.8296 mL
100 mM 0.0265 mL 0.1327 mL 0.2655 mL 0.6637 mL
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Prochloraz Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prochloraz67747-09-5BTS 40542BTS40542BTS-40542FungalEstrogen Receptor/ERRAndrogen ReceptorAryl Hydrocarbon ReceptorAhRimidazoleantifungalergosterolbiosynthesishumanplacentamicrosomalaromatase,estrogenERandrogenarylhydrocarbonInhibitorinhibitorinhibit

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