Pyrazinamide (Synonyms: Pyrazinecarboxamide; Pyrazinoic acid amide)

Name: Pyrazinamide
Brand: MedChemExpress
SKU: HY-B0271
Rating: 5.0 (2 reviews)

Cat. No.: HY-B0271 Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pyrazinamide (Pyrazinecarboxamide; Pyrazinoic acid amide) is a potent and orally active antitubercular antibiotic. Pyrazinamide is a proagent that is converted to the active form pyrazinoic acid (POA) by PZase/nicotinamidase encoded by the pncA gene in M. tuberculosis.

For research use only. We do not sell to patients.

Pyrazinamide Chemical Structure

CAS No. : 98-96-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
10 g	USD 66	In-stock	Estimated Time of Arrival: December 31
50 g	USD 158	In-stock	Estimated Time of Arrival: December 31
> 100 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Pyrazinamide:

2 Publications Citing Use of MCE Pyrazinamide

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: Mycobacterium tuberculosis^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	> 1 x 10⁴ μM Compound: PZA	Cytotoxicity against human HepG2 cells after 24 hrs Cytotoxicity against human HepG2 cells after 24 hrs	[PMID: 24388809]
Vero	IC₅₀	> 50 μg/mL Compound: Pyrazinamide	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24044875]
Vero	IC₅₀	> 62.5 μg/mL Compound: Pyrazinamide	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24721315]

In Vitro

Pyrazinamide exhibits remarkable activity in vivo activity, has no activity against growing Mycobacterium tuberculosis except at an acidic pH. In M. tuberculosis, acidic pH enhances the intracellular accumulation of pyrazinoic acid (POA), the active derivative of PZA.
POA is thought to disrupt membrane energetics and inhibit membrane transport function at acid pH in?Mycobacterium tuberculosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

123.11

Formula

C₅H₅N₃O

CAS No.

98-96-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NC=CN=C1)N

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (406.14 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 6.67 mg/mL (54.18 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.1228 mL	40.6141 mL	81.2282 mL
5 mM	1.6246 mL	8.1228 mL	16.2456 mL
10 mM	0.8123 mL	4.0614 mL	8.1228 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (20.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 27.5 mg/mL (223.38 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (150 KB) LCMS (88 KB) GC (246 KB)

Handling Instructions (2659 KB)

References

[1]. Y Zhang, et al. Role of acid pH and deficient efflux of pyrazinoic acid in unique susceptibility of Mycobacterium tuberculosis to pyrazinamide. J Bacteriol. 1999 Apr;181(7):2044-9. [Content Brief]

[2]. Ying Zhang, et al. Mechanisms of Pyrazinamide Action and Resistance. Microbiol Spectr. 2014 Aug;2(4):MGM2-0023-2013. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.1228 mL	40.6141 mL	81.2282 mL	203.0704 mL
	5 mM	1.6246 mL	8.1228 mL	16.2456 mL	40.6141 mL
	10 mM	0.8123 mL	4.0614 mL	8.1228 mL	20.3070 mL
	15 mM	0.5415 mL	2.7076 mL	5.4152 mL	13.5380 mL
	20 mM	0.4061 mL	2.0307 mL	4.0614 mL	10.1535 mL
	25 mM	0.3249 mL	1.6246 mL	3.2491 mL	8.1228 mL
	30 mM	0.2708 mL	1.3538 mL	2.7076 mL	6.7690 mL
	40 mM	0.2031 mL	1.0154 mL	2.0307 mL	5.0768 mL
	50 mM	0.1625 mL	0.8123 mL	1.6246 mL	4.0614 mL
DMSO	60 mM	0.1354 mL	0.6769 mL	1.3538 mL	3.3845 mL
	80 mM	0.1015 mL	0.5077 mL	1.0154 mL	2.5384 mL
	100 mM	0.0812 mL	0.4061 mL	0.8123 mL	2.0307 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Pyrazinamide Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyrazinamide98-96-4Pyrazinecarboxamide Pyrazinoic acid amideBacterialAutophagyAntibioticAntitubercularPOAPZase/nicotinamidasepncA geneM. tuberculosisMycobacterium tuberculosisInhibitorinhibitorinhibit

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Pyrazinamide (Synonyms: Pyrazinecarboxamide; Pyrazinoic acid amide)

Customer Review

Other Forms of Pyrazinamide:

Pyrazinamide Related Antibodies

2 Publications Citing Use of MCE Pyrazinamide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Pyrazinamide Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Pyrazinamide Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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